| Literature DB >> 24233000 |
Abstract
Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequent cell divisions lead to the formation of colonies showing an early differentiation of small meristematic cells and large vacuolated "suspensor-type" cells.Entities:
Year: 1989 PMID: 24233000 DOI: 10.1007/BF00716854
Source DB: PubMed Journal: Plant Cell Rep ISSN: 0721-7714 Impact factor: 4.570