Literature DB >> 24232994

Transformed calli obtained by direct gene transfer into sunflower protoplasts.

A L Moyne1, D Tagu, V Thor, C Bergounioux, G Freyssinet, P Gadal.   

Abstract

Helianthus annuus protoplasts were transformed with the plasmid pCaMVNEO (Frommet al. 1986) conferring kanamycin resistance to plant. Transformed calli were selected with a frequency of 4 calli for 10(6) treated protoplasts. DNA was extracted from kanamycin resistant calli. Analysis of this DNA shows the presence of the NPTII gene.

Entities:  

Year:  1989        PMID： 24232994     DOI： 10.1007/BF00716848

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  7 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Stable transformation of maize after gene transfer by electroporation.

Authors:  M E Fromm; L P Taylor; V Walbot
Journal:  Nature       Date:  1986 Feb 27-Mar 5       Impact factor: 49.962

3.  Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.

Authors:  R D Shillito; J Paszkowski; I Potrykus
Journal:  Plant Cell Rep       Date:  1983-10       Impact factor: 4.570

4.  Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors:  I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal:  Plant Mol Biol       Date:  1987-09       Impact factor: 4.076

5.  Callus and embryoid formation from protoplasts of Helianthus annuus.

Authors:  A L Moyne; V Thor; B Pelissier; C Bergounioux; G Freyssinet; P Gadal
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570

6.  Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium.

Authors:  J J Finer
Journal:  Plant Cell Rep       Date:  1987-10       Impact factor: 4.570

7.  Direct gene transfer to plants.

Authors:  J Paszkowski; R D Shillito; M Saul; V Mandák; T Hohn; B Hohn; I Potrykus
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598
  7 in total
  2 in total

1.  Studies on plant regeneration from protoplasts in the genus Helianthus.

Authors:  C Chanabe; M Burrus; D Bidney; G Alibert
Journal:  Plant Cell Rep       Date:  1991-03       Impact factor: 4.570

2.  Transformation of sunflower (Helianthus annuus L.): a reliable protocol.

Authors:  N Knittel; V Gruber; G Hahne; P Lénée
Journal:  Plant Cell Rep       Date:  1994-12       Impact factor: 4.570
  2 in total

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