Literature DB >> 24232845

Preparation and characterization of monoclonal antibodies which recognise different gibberellin epitopes.

J P Knox1, M H Beale, G W Butcher, J Macmillan.   

Abstract

The production and characterization of high-affinity monoclonal antibodies (McAb) to gibberellins (GAs) is reported. Hybrid myelomas were derived from immunisations with conjugates in which immunogenic proteins were linked to GA1 at carbon-3 and to GA4 and GA9 at carbon-17. A series of McAb which display specificities allowing recognition of, and the discrimination between GA1, GA20, GA4 and GA9 is described. These McAb can be used in quantitative immunoassays for underivatised GAs.

Entities:  

Year:  1987        PMID： 24232845     DOI： 10.1007/BF00392384

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  10 in total

1.  The immunoassay of gibberellins : II. Quantitation of GA3, GA 4 and GA 7 by ultra-sensitive solid-phase enzyme immunoassays.

Authors:  R Atzorn; E W Weiler
Journal:  Planta       Date:  1983-01       Impact factor: 4.116

2.  Immunological assay for plant hormones using specific antibodies to indoleacetic acid and gibberellic acid.

Authors:  S Fuchs; Y Fuchs
Journal:  Biochim Biophys Acta       Date:  1969-12-30

3.  Immunological studies of plant hormones. Detection and estimation by immunological assays.

Authors:  S Fuchs; J Haimovich; Y Fuchs
Journal:  Eur J Biochem       Date:  1971-02-01

4.  Determination of affinity and specificity of anti-hapten antibodies by competitive radioimmunoassay.

Authors:  R Müller
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

5.  Monoclonal antibody screening: two methods using antigens immobilized on nitrocellulose.

Authors:  E Smith; K Roberts; G W Butcher; G Galfre
Journal:  Anal Biochem       Date:  1984-04       Impact factor: 3.365

6.  A dot-immunobinding assay for monoclonal and other antibodies.

Authors:  R Hawkes; E Niday; J Gordon
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

7.  Preparation of monoclonal antibodies: strategies and procedures.

Authors:  G Galfrè; C Milstein
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

8.  The immunoassay of gibberellins : I. radioimmunoassays for the gibberellins A1, A 3, A 4, A 7, A 9 and A 20.

Authors:  R Atzorn; E W Weiler
Journal:  Planta       Date:  1983-01       Impact factor: 4.116

9.  Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.

Authors:  G Galfrè; C Milstein; B Wright
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

10.  Determination of femtomol quantities of gibberellic acid by radioimmunoassay.

Authors:  E W Weiler; U Wieczorek
Journal:  Planta       Date:  1981-06       Impact factor: 4.116
  10 in total
  4 in total

1.  cDNA cloning of a tetraubiquitin gene, and expression of ubiquitin-containing transcripts, in aleurone layers of Avena fatua.

Authors:  G J Reynolds; R Hooley
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

2.  Gibberellin perception at the plasma membrane of Avena fatua aleurone protoplasts.

Authors:  R Hooley; M H Beale; S J Smith
Journal:  Planta       Date:  1991-01       Impact factor: 4.116

3.  Immunochromatographic purification of gibberellins from vegetative tissues of Cucumis sativus L: Separation and identification of 13-hydroxy and 13-deoxy gibberellins.

Authors:  V A Smith; V M Sponsel; C Knatt; P Gaskin; J Macmillan
Journal:  Planta       Date:  1991-11       Impact factor: 4.116

4.  Development of a Monoclonal Antibody-Based icELISA for the Detection of Ustiloxin B in Rice False Smut Balls and Rice Grains.

Authors:  Xiaoxiang Fu; Ali Wang; Xiaohan Wang; Fengke Lin; Lishan He; Daowan Lai; Yang Liu; Qing X Li; Ligang Zhou; Baoming Wang
Journal:  Toxins (Basel)       Date:  2015-08-28       Impact factor: 4.546
  4 in total

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