Literature DB >> 2422978

A biological assay for epidermal growth factor/urogastrone and related polypeptides.

G Carpenter, J Zendegui.   

Abstract

A bioassay for epidermal growth factor (EFG) which is rapid, specific, and extremely sensitive is described. As the bioassay detects as little as 25 pg of EGF, this assay is more sensitive than commonly used radioreceptor assays and nearly as sensitive as radioimmunoassays. This bioassay involves only the measurement of the proliferation of cultures of an EGF-requiring cell line and can be carried out in a quantitative manner over a 40-fold range of EGF concentrations.

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Year:  1986        PMID: 2422978     DOI: 10.1016/0003-2697(86)90093-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  5 in total

1.  Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins.

Authors:  M Shoyab; V L McDonald; C Byles; G J Todaro; G D Plowman
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

2.  Recombinant human epidermal growth factor precursor is a glycosylated membrane protein with biological activity.

Authors:  B Mroczkowski; M Reich; K Chen; G I Bell; S Cohen
Journal:  Mol Cell Biol       Date:  1989-07       Impact factor: 4.272

3.  Reduced growth-factor requirement of keloid-derived fibroblasts may account for tumor growth.

Authors:  S B Russell; K M Trupin; S Rodríguez-Eaton; J D Russell; J S Trupin
Journal:  Proc Natl Acad Sci U S A       Date:  1988-01       Impact factor: 11.205

4.  Reinitiation of DNA synthesis in quiescent mouse keratinocytes; regulation by polypeptide hormones, cholera toxin, dexamethasone, and retinoic acid.

Authors:  M Reiss; C L Dibble
Journal:  In Vitro Cell Dev Biol       Date:  1988-06

5.  Isolation and characterization of a newly identified endothelial cell mitogen produced by AtT-20 cells.

Authors:  J Plouët; J Schilling; D Gospodarowicz
Journal:  EMBO J       Date:  1989-12-01       Impact factor: 11.598

  5 in total

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