Literature DB >> 24228875

Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein.

E S Kotova1, I V Sorokina, S B Akopov, L G Nikolaev, E D Sverdlov.   

Abstract

The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.

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Year:  2013        PMID： 24228875     DOI： 10.1134/S0006297913080038

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  2 in total

1.  The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes.

Authors:  Ekaterina S Gushchanskaya; Artem V Artemov; Sergey V Ulyanov; Maria D Logacheva; Aleksey A Penin; Elena S Kotova; Sergey B Akopov; Lev G Nikolaev; Olga V Iarovaia; Eugene D Sverdlov; Alexey A Gavrilov; Sergey V Razin
Journal:  Epigenetics       Date:  2014-04-15       Impact factor: 4.528

2.  Binding of Protein Factor CTCF within Chicken Genome Alpha-Globin Locus.

Authors:  E S Kotova; S B Akopov; D A Didych; N V Petrova; O V Iarovaia; S V Razin; L G Nikolaev
Journal:  Acta Naturae       Date:  2016 Jan-Mar       Impact factor: 1.845
  2 in total

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