Literature DB >> 24227552

Evaluation of membrane adsorption-epifluorescence microscopy for the enumeration of bacteria in coastal surface films.

L M Sewell1, G Bitton, J S Bays.   

Abstract

We have evaluated a method for enumerating surface slick bacteria by combining a membrane adsorption procedure with epifluorescence microscopy. Various chemicals were investigated for their ability to enhance bacterial elution from the membrane filters. The results of the elution-epifluorescence method were compared to plate counts and to direct epifluorescence counts of the sampling membrane filters. In all tests, the elution-epifluorescence technique yielded significantly higher bacterial concentrations.

Year:  1981        PMID： 24227552     DOI： 10.1007/BF02341431

Source DB:  PubMed          Journal:  Microb Ecol        ISSN： 0095-3628            Impact factor:   4.552


  3 in total

1.  Enrichment and association of bacteria and particulates in salt marsh surface water.

Authors:  R W Harvey; L Y Young
Journal:  Appl Environ Microbiol       Date:  1980-04       Impact factor: 4.792

2.  Use of nuclepore filters for counting bacteria by fluorescence microscopy.

Authors:  J E Hobbie; R J Daley; S Jasper
Journal:  Appl Environ Microbiol       Date:  1977-05       Impact factor: 4.792

3.  New sampling device for the recovery of petroleum hydrocarbons and fatty acids from aqueous surface films.

Authors:  R Miget; H Kator; C Oppenheimer; J L Laseter; E J Ledet
Journal:  Anal Chem       Date:  1974-07       Impact factor: 6.986
  3 in total
  2 in total

1.  A membrane adsorption-SEM technique for observing neuston organisms.

Authors:  J S Maki; C C Remsen
Journal:  Microb Ecol       Date:  1983-07       Impact factor: 4.552

2.  Halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality.

Authors:  Henry N Williams; Despoina S Lymperopoulou; Rana Athar; Ashvini Chauhan; Tamar L Dickerson; Huan Chen; Edward Laws; Timkhite-Kulu Berhane; Adrienne R Flowers; Nadine Bradley; Shanterial Young; Denene Blackwood; Jacqueline Murray; Oladipupo Mustapha; Cory Blackwell; Yahsuan Tung; Rachel T Noble
Journal:  ISME J       Date:  2015-08-07       Impact factor: 10.302
  2 in total

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