Phenylpropenoid phenolics in sweetbay magnolia as chemical determinants of host use in saturniid silkmoths (Callosamia).

Host plant chemistry can play an important role in determining the evolution of host use patterns in herbivorous insects by influencing host selection, consumption, and assimilation of foliage. We used a comparative approach to test the hypothesis that specialist herbivores of sweetbay magnolia (Magnolia virginiana) possess adaptations that allow them to overcome chemical deterrents or toxins that prevent herbivory by unadapted herbivores. The three silkmoth species in the genusCallosamia can be collectively regarded as specialists on magnoliaceous hosts; however, only the monophagousC. securifera is able to complete development on sweetbay magnolia, its natural host. In laboratory assays with intact foliage, bothC. angulifera and the polyphagousC. promethea fed readily on sweetbay but were unable to survive past the third instar. Two neolignan compounds, magnolol and a biphenyl ether, were found to reduce neonate growth and survival of unadapted herbivore species when painted on acceptable host leaves at concentrations similar to those found in sweetbay foliage. Both compounds significantly reduced neonate growth ofC. angulifera andC. promethea but had no effect on the sweetbay specialist,C. securifera, indicating that the latter species possesses the unique ability in the genus to tolerate, metabolize, or otherwise circumvent the phytochemical defenses of this host.