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Literature DB >> 24227140

A simple, nondestructive spraying assay for the detection of an active kanamycin resistance gene in transgenic tomato plants.

Abstract

A simple, nondestructive kanamycin spraying assay for detecting neomycin phosphotransferase II activity in tomato has been developed. This assay does not require the use of tissue culture or biochemical methods, but allows transgenic and non-transgenic tomato plants to be distinguished directly at the plant level in the green-house. Its potential applications in large-scale genetic analyses are discussed.

Entities: Chemical Species

Year: 1989 PMID： 24227140 DOI： 10.1007/BF00288794

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

5 in total

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Journal: Plant Mol Biol Date: 1985-05 Impact factor: 4.076

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Authors: R B Horsch; R T Fraley; S G Rogers; P R Sanders; A Lloyd; N Hoffmann
Journal: Science Date: 1984-02-03 Impact factor: 47.728

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Authors: B Reiss; R Sprengel; H Will; H Schaller
Journal: Gene Date: 1984-10 Impact factor: 3.688

4. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.

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Journal: Plant Cell Rep Date: 1986-04 Impact factor: 4.570

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Authors: P H Schreier; E A Seftor; J Schell; H J Bohnert
Journal: EMBO J Date: 1985-01 Impact factor: 11.598

5 in total

16 in total

1. Cre recombinase expression can result in phenotypic aberrations in plants.

Authors: Eric R Coppoolse; Marianne J de Vroomen; Dick Roelofs; Jaap Smit; Femke van Gennip; Bart J M Hersmus; H John J Nijkamp; Mark J J van Haaren
Journal: Plant Mol Biol Date: 2003-01 Impact factor: 4.076

Review 2. Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.

Authors: I M G Padilla; L Burgos
Journal: Plant Cell Rep Date: 2010-07-20 Impact factor: 4.570

3. Size does matter: cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites.

Authors: Eric R Coppoolse; Marianne J de Vroomen; Femke van Gennip; Bart J M Hersmus; Mark J J van Haaren
Journal: Plant Mol Biol Date: 2005-07 Impact factor: 4.076

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Authors: A Burbidge; T M Grieve; K J Woodman; I B Taylor
Journal: Theor Appl Genet Date: 1995-11 Impact factor: 5.699

5. Stable expression of the GUS reporter gene in chrysanthemum depends on binary plasmid T-DNA.

Authors: J de Jong; M M Mertens; W Rademaker
Journal: Plant Cell Rep Date: 1994-11 Impact factor: 4.570

6. Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype.

Authors: E Jacobsen; P Reinhout; J E Bergervoet; J de Looff; P E Abidin; D J Huigen; M S Ramanna
Journal: Theor Appl Genet Date: 1992-11 Impact factor: 5.699

10. T-DNA-tagged chromosome 12 in donor Lycopersicon esculentum × L. pennellii is retained in asymmetric somatic hybrids with recipient Solanum lycopersicoides.

Authors: P F McCabe; L J Dunbar; A Guri; K C Sink
Journal: Theor Appl Genet Date: 1993-04 Impact factor: 5.699