Literature DB >> 24226078

Vacuolar pH in radish cotyledonal mesophyll cells.

D Strack1, V Sharma, H Felle.   

Abstract

The vacuolar pH in cotyledonal mesophyll cells from radish (Raphanus sativus L. var. sativus) seedlings was determined from vacuoles, isolated from protoplasts through osmotic shock, by means of measurement of vacuole extracts with a pH meter and the "methylamine method", and gave mean pH values of 6.28 and 6.26, respectively. Direct in situ measurements of the vacuolar pH from intact leaf tissue were recorded with pH-sensitive microelectrodes and gave a mean value of 6.0. The results are discussed with respect to possible erroneous pH measurements and the vacuolar location of specific anabolic reactions.

Entities:  

Year:  1987        PMID： 24226078     DOI： 10.1007/BF00393875

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  10 in total

1.  Determination of pH in chloroplasts. 3. Ammonium uptake as a measure of pH in chloroplasts and sub-chloroplast particles.

Authors:  H Rottenberg; T Grunwald
Journal:  Eur J Biochem       Date:  1972-01-31

2.  The inner membrane of the chloroplast envelope as the site of specific metabolite transport.

Authors:  H W Heldt; F Sauer
Journal:  Biochim Biophys Acta       Date:  1971-04-06

3.  Vacuolar pH Measurement in Higher Plant Cells : I. EVALUATION OF THE METHYLAMINE METHOD.

Authors:  A Kurkdjian; J Guern
Journal:  Plant Physiol       Date:  1981-05       Impact factor: 8.340

4.  Conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by isolated vacuoles of Pisum sativum L.

Authors:  M Guy; H Kende
Journal:  Planta       Date:  1984-03       Impact factor: 4.116

5.  Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue.

Authors:  W Lin; G J Wagner; H W Siegelman; G Hind
Journal:  Biochim Biophys Acta       Date:  1977-02-14

6.  Amine Transport in Riccia fluitans: Cytoplasmic and Vacuolar pH Recorded by a pH-Sensitive Microelectrode.

Authors:  A Bertl; H Felle; F W Bentrup
Journal:  Plant Physiol       Date:  1984-09       Impact factor: 8.340

7.  pH in Vacuoles Isolated from Castor Bean Endosperm.

Authors:  M Nishimura
Journal:  Plant Physiol       Date:  1982-09       Impact factor: 8.340

8.  Tissue distribution of phenylpropanoid metabolism in cotyledons of Raphanus sativus L.

Authors:  D Strack; M Pieroth; H Scharf; V Sharma
Journal:  Planta       Date:  1985-07       Impact factor: 4.116

9.  Development of 1-O-sinapoyl-β-D-glucose: L-malate sinapoyltransferase activity in cotyledons of red radish (Raphanus sativus L. var. sativus).

Authors:  D Strack
Journal:  Planta       Date:  1982-06       Impact factor: 4.116

10.  Localization of Fructan Metabolism in the Vacuoles Isolated from Protoplasts of Jerusalem Artichoke Tubers (Helianthus tuberosus L.).

Authors:  M Frehner; F Keller; A Wiemken
Journal:  J Plant Physiol       Date:  2012-01-20       Impact factor: 3.549
  10 in total
  2 in total

1.  Purification and characterization of sinapoylglucose:malate sinapoyltransferase from Raphanus sativus L.

Authors:  W Gräwe; P Bachhuber; H P Mock; D Strack
Journal:  Planta       Date:  1992-05       Impact factor: 4.116

2.  Mapping membrane potential differences and dye-coupling in internodal tissues of tomato (Solanum lycopersicum L.).

Authors:  C van der Schoot; A J van Bel
Journal:  Planta       Date:  1990-08       Impact factor: 4.116
  2 in total

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