Literature DB >> 2422496

Large-section cryomicrotomy for in vitro receptor autoradiography.

P G Gillberg, S S Jossan, H Askmark, S M Aquilonius.   

Abstract

A method for processing human postmortem material for application of autoradiography to large cryosections is described. Whole organ or tissue slices 5 mm thick are frozen between copper plates cooled with liquid nitrogen. They are subsequently embedded in carboxymethyl-cellulose, and 40- to 80-microns sections are taken up on adhesive tape and paper and transferred to gelatinized glass plates. Two procedures for incubation of large sections are described. The method is exemplified by autoradiographs of human brain hemisphere ([ 3H]quinuclidinylbenzilate) and whole biceps muscle ([ 3H]alpha-bungarotoxin).

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Year:  1986        PMID： 2422496     DOI： 10.1016/0160-5402(86)90065-3

Source DB:  PubMed          Journal:  J Pharmacol Methods        ISSN： 0160-5402


  9 in total

1.  Increased binding of 3H-L-deprenyl in spinal cords from patients with amyotrophic lateral sclerosis as demonstrated by autoradiography.

Authors:  S M Aquilonius; S S Jossan; J G Ekblom; H Askmark; P G Gillberg
Journal:  J Neural Transm Gen Sect       Date:  1992

2.  Receptor alterations in manganese intoxicated monkeys.

Authors:  H Eriksson; P G Gillberg; S M Aquilonius; K G Hedström; E Heilbronn
Journal:  Arch Toxicol       Date:  1992       Impact factor: 5.153

3.  Localization of monoamine oxidase B in human brain by autoradiographical use of 11C-labelled L-deprenyl.

Authors:  S S Jossan; R d'Argy; P G Gillberg; S M Aquilonius; B Långström; C Halldin; P Bjurling; C G Stålnacke; J Fowler; R MacGregor
Journal:  J Neural Transm       Date:  1989       Impact factor: 3.575

4.  Distribution of galanin-binding sites in the monkey and human telencephalon: preliminary observations.

Authors:  C Köhler; A Persson; T Melander; E Theodorsson; G Sedvall; T Hökfelt
Journal:  Exp Brain Res       Date:  1989       Impact factor: 1.972

5.  ³H-deprenyl and ³H-PIB autoradiography show different laminar distributions of astroglia and fibrillar β-amyloid in Alzheimer brain.

Authors:  Amelia Marutle; Per-Göran Gillberg; Assar Bergfors; Wenfeng Yu; Ruiqing Ni; Inger Nennesmo; Larysa Voytenko; Agneta Nordberg
Journal:  J Neuroinflammation       Date:  2013-07-23       Impact factor: 8.322

6.  Visualization of regional tau deposits using (3)H-THK5117 in Alzheimer brain tissue.

Authors:  Laetitia Lemoine; Laure Saint-Aubert; Amelia Marutle; Gunnar Antoni; Jonas P Eriksson; Bernardino Ghetti; Nobuyuki Okamura; Inger Nennesmo; Per-Göran Gillberg; Agneta Nordberg
Journal:  Acta Neuropathol Commun       Date:  2015-07-02       Impact factor: 7.801

7.  Cortical laminar tau deposits and activated astrocytes in Alzheimer's disease visualised by 3H-THK5117 and 3H-deprenyl autoradiography.

Authors:  Laetitia Lemoine; Laure Saint-Aubert; Inger Nennesmo; Per-Göran Gillberg; Agneta Nordberg
Journal:  Sci Rep       Date:  2017-04-04       Impact factor: 4.379

8.  Synthesis and Autoradiography of Novel F-18 Labeled Reversible Radioligands for Detection of Monoamine Oxidase B.

Authors:  Sangram Nag; Zhisheng Jia; Marie Svedberg; Alex Jackson; Rabia Ahmad; Sajinder Luthra; Katarina Varnäs; Lars Farde; Christer Halldin
Journal:  ACS Chem Neurosci       Date:  2020-12-07       Impact factor: 4.418

9.  Development of 11C-Labeled ASEM Analogues for the Detection of Neuronal Nicotinic Acetylcholine Receptors (α7-nAChR).

Authors:  Sangram Nag; Patricia Miranda-Azpiazu; Zhisheng Jia; Prodip Datta; Ryosuke Arakawa; Mohammad Mahdi Moein; Zhou Yang; Yaoquan Tu; Laetitia Lemoine; Hans Ågren; Agneta Nordberg; Bengt Långström; Christer Halldin
Journal:  ACS Chem Neurosci       Date:  2022-01-12       Impact factor: 5.780
  9 in total

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