| Literature DB >> 24221551 |
L A Van Asselt1, R J Timmers, H J Goos, J G Lambert, P G Van Oordt.
Abstract
Isolated gonadotrops of the African catfish,Clarias gariepinus, were incubated with dopamine (DA) and/or catecholestrone and the activity of the enzyme catechol-O-methyltransferase (COMT) was determined by measuring the methylated products. From the apparent Km values for DA and catecholestrone of 0.4-1.3 μM and 17.9-25.2 μM respectively, it was concluded that catecholestrone is a better substrate for the enzyme COMT, compared to DA. Moreover, the methylation of DA is inhibited by comparatively low concentrations of catecholestrone.Entities:
Year: 1989 PMID: 24221551 DOI: 10.1007/BF01875023
Source DB: PubMed Journal: Fish Physiol Biochem ISSN: 0920-1742 Impact factor: 2.794