Literature DB >> 24221434

Isolation and characterization of two middle repetitive DNA sequences of nuclear tobacco genome.

V Kuhrová1, M Bezděk, B Vyskot, B Koukalová, J Fajkus.   

Abstract

Two DNA sequences, R8.1 and R8.3, representing two distinct classes of tobacco genomic repeated DNA, were cloned and characterized by Southern blot analysis. Both R8.1 and R8.3 were found to be homologous to the Nicotiana tomentosiformis component of the allotetraploid Nicotiana tabacum genome, and each of them represents about 0.3% of nuclear DNA. The R8.1 and R8.3 differ in the mode of distribution in chromosomes, as revealed by in situ DNA/DNA hybridization.

Entities:  

Year:  1991        PMID： 24221434     DOI： 10.1007/BF00224983

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  5 in total

1.  Isolation and sequencing of HRS60dim1, a dimeric member of the HRS60-family of a tobacco DNA repeat.

Authors:  R Matyásek; B oukalová; J Reich
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Lambda replacement vectors carrying polylinker sequences.

Authors:  A M Frischauf; H Lehrach; A Poustka; N Murray
Journal:  J Mol Biol       Date:  1983-11-15       Impact factor: 5.469

4.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  A BamHI family of highly repeated DNA sequences of Nicotiana tabacum.

Authors:  B Koukalová; J Reich; R Matyášek; V Kuhrová; M Bezděk
Journal:  Theor Appl Genet       Date:  1989-07       Impact factor: 5.699
  5 in total
  6 in total

1.  Molecular analysis of highly repeated genome fractions in Solanum and their use as markers for the characterization of species and cultivars.

Authors:  G Schweizer; N Borisjuk; L Borisjuk; M Stadler; T Stelzer; L Schilde; V Hemleben
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

2.  Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics.

Authors:  A Kenton; A S Parokonny; Y Y Gleba; M D Bennett
Journal:  Mol Gen Genet       Date:  1993-08

3.  Characterization of a new family of tobacco highly repetitive DNA, GRS, specific for the Nicotiana tomentosiformis genomic component.

Authors:  B Gazdová; J Siroký; J Fajkus; B Brzobohatý; A Kenton; A Parokonny; J S Heslop-Harrison; K Palme; M Bezdĕk
Journal:  Chromosome Res       Date:  1995-06       Impact factor: 5.239

4.  Studies on the effects of a flanking repetitive sequence on the expression of single-copy transgenes in Nicotiana sylvestris and in N. sylvestris-N. tomentosiformis hybrids.

Authors:  Christian Kunz; Jarunya Narangajavana; Johannes Jakowitsch; Young-Doo Park; T René Delon; Ales Kovarik; Blazena Koukalová; Johannes van der Winden; Eduardo Moscone; Werner Aufsatz; M Florian Mette; Marjori Matzke; Antonius J M Matzke
Journal:  Plant Mol Biol       Date:  2003-05       Impact factor: 4.076

5.  Physiological aspects of genome variability in tissue culture. II. Growth phase-dependent quantitative variability of repetitive BstNI fragments of primary cultures of Daucus carota L.

Authors:  B Arnholdt-Schmitt
Journal:  Theor Appl Genet       Date:  1995-10       Impact factor: 5.699

6.  Comparative analysis of DNA methylation in tobacco heterochromatic sequences.

Authors:  A Kovarík; B Koukalová; K Y Lim; R Matyásek; C P Lichtenstein; A R Leitch; M Bezdek
Journal:  Chromosome Res       Date:  2000       Impact factor: 4.620
  6 in total

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