Literature DB >> 2422138

Partial copurification of scrapie-associated fibrils and scrapie infectivity.

R A Somerville, P A Merz, R I Carp.   

Abstract

The association between scrapie infectivity and scrapie-associated fibrils (SAF) during a partial purification procedure for infectivity was investigated. Scrapie infectivity and SAF can be separated from most membrane components by subcellular fractionation of infected mouse brain to obtain a synaptosomal fraction, followed by detergent treatment and density gradient centrifugation. After different detergent treatments, with either octyl glucoside or sodium N-lauroyl sarcosinate, SAF showed differing sedimentation characteristics but nevertheless cosedimented with scrapie infectivity in both cases. Copurification under two different conditions provides more evidence that SAF may be a form of the infectious agent of scrapie.

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Year:  1986        PMID： 2422138     DOI： 10.1159/000149654

Source DB:  PubMed          Journal:  Intervirology        ISSN： 0300-5526            Impact factor:   1.763


  9 in total

1.  Immunological comparison of scrapie-associated fibrils isolated from animals infected with four different scrapie strains.

Authors:  R J Kascsak; R Rubenstein; P A Merz; R I Carp; N K Robakis; H M Wisniewski; H Diringer
Journal:  J Virol       Date:  1986-09       Impact factor: 5.103

2.  Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie.

Authors:  L J van Keulen; B E Schreuder; R H Meloen; G Mooij-Harkes; M E Vromans; J P Langeveld
Journal:  J Clin Microbiol       Date:  1996-05       Impact factor: 5.948

3.  Synthetic scrapie infectivity: interaction between recombinant PrP and scrapie brain-derived RNA.

Authors:  Steve Simoneau; Achim Thomzig; Marie-Madeleine Ruchoux; Nicolas Vignier; Martin L Daus; Anna Poleggi; Pierre Lebon; Sophie Freire; Valerie Durand; Silvia Graziano; Roberta Galeno; Franco Cardone; Emmanuel Comoy; Maurizio Pocchiari; Michael Beekes; Jean-Philippe Deslys; Jean-Guy Fournier
Journal:  Virulence       Date:  2015-01-13       Impact factor: 5.882

4.  Astrocyte gene expression in Creutzfeldt-Jakob disease.

Authors:  L Manuelidis; D M Tesin; T Sklaviadis; E E Manuelidis
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

5.  Early spread of scrapie from the gastrointestinal tract to the central nervous system involves autonomic fibers of the splanchnic and vagus nerves.

Authors:  P A McBride; W J Schulz-Schaeffer; M Donaldson; M Bruce; H Diringer; H A Kretzschmar; M Beekes
Journal:  J Virol       Date:  2001-10       Impact factor: 5.103

6.  Analyses of frequency of infection, specific infectivity, and prion protein biosynthesis in scrapie-infected neuroblastoma cell clones.

Authors:  R E Race; B Caughey; K Graham; D Ernst; B Chesebro
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

7.  The association between PrP and infectivity in scrapie and BSE infected mouse brain.

Authors:  R A Somerville; A J Dunn
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

8.  Antisera to scrapie-associated fibril protein and prion protein decorate scrapie-associated fibrils.

Authors:  P A Merz; R J Kascsak; R Rubenstein; R I Carp; H M Wisniewski
Journal:  J Virol       Date:  1987-01       Impact factor: 5.103

9.  The major polypeptide of scrapie-associated fibrils (SAF) has the same size, charge distribution and N-terminal protein sequence as predicted for the normal brain protein (PrP).

Authors:  J Hope; L J Morton; C F Farquhar; G Multhaup; K Beyreuther; R H Kimberlin
Journal:  EMBO J       Date:  1986-10       Impact factor: 11.598
  9 in total

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