The effect of salinity, temperature and time on the accumulation and depuration of copper in ragworm, Hediste (Nereis) diversicolor (O.F. Muller).

Under a low temperature condition of 12°C, accumulation of copper by the ragworm, Hediste (Nereis) diversicolor, increased at low salinity of 7.6‰ but was reduced at intermediate and high salinities of 15.25 and 30.5‰, respectively. Copper contents of the worms at low salinity and temperature ranged from 68 to 185 µg/g. Under increasing temperature of 17 and 22°C, bioavailability of copper to the worms increased irrespective of salinity gradient. The copper contents of the worm ranged from 59 to 784 µg/g dry weight. ANOVA analysis of data showed that salinity did not significantly affect accumulation of copper by the worm. Temperature significantly affected accumulation. The interaction of salinity and temperature was significant and complex, and days of exposure did not influence accumulation. Depuration of copper by the worm was independent of salinity which ranged from 510 to 15.6 µg/g. Temperature significantly affected depuration. The interaction of salinity and temperature was significant. However, individual worms neither accumulated nor depurated at the same rate.