Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat.

Literature DB >> 24221271

A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat.

Abstract

An unusual genomic DNA clone, PSR454, was isolated from a partial genomic library of wheat. This sequence is moderately repeated and detects at least 30 related sequences, all located in a tight linkage block on the long arm of chromosome 3B. When used as a RFLP probe, PSR454 detects a high level of polymorphism between wheat varieties that carry the sequence. There is no detectable hybridisation to sequences in one-third of the varieties tested, providing an "on-off" polymorphism that can be detected on dot blots, rather than the more resource-consuming conventional Southern analysis.

Entities: Species

Year: 1991 PMID： 24221271 DOI： 10.1007/BF00228682

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

7 in total

1. Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.).

Authors: B S Landry; R Kesseli; H Leung; R W Michelmore
Journal: Theor Appl Genet Date: 1987-09 Impact factor: 5.699

2. The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm.

Authors: P J Sharp; S Chao; S Desai; M D Gale
Journal: Theor Appl Genet Date: 1989-09 Impact factor: 5.699

7. The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis.

Authors: W Y Cheung; M D Gale
Journal: Plant Mol Biol Date: 1990-05 Impact factor: 4.076

7 in total

6 in total

Review 4. Comparative genetics in the grasses.

Authors: K M Devos; M D Gale
Journal: Plant Mol Biol Date: 1997-09 Impact factor: 4.076

5. Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects.

Authors: T Yang; G Segal; S Abbo; M Feldman; H Fromm
Journal: Mol Gen Genet Date: 1996-10-28

6. Isolation of two differentially expressed wheat ACC synthase cDNAs and the characterization of one of their genes with root-predominant expression.

Authors: K Subramaniam; S Abbo; P P Ueng
Journal: Plant Mol Biol Date: 1996-08 Impact factor: 4.076

6 in total

A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat.

1. Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.).

2. The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm.

3. RFLP-based genetic maps of wheat homoeologous group 7 chromosomes.

4. Evaluation of random cDNA clones as probes for human restriction fragment polymorphisms.

5. Molecular mapping of rice chromosomes.

6. Studies on transformation of Escherichia coli with plasmids.

7. The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis.

1. Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley.

2. RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye.

3. Detection of DNA sequence polymorphisms among wheat varieties.

Review 4. Comparative genetics in the grasses.

5. Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects.

6. Isolation of two differentially expressed wheat ACC synthase cDNAs and the characterization of one of their genes with root-predominant expression.