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Literature DB >> 24220785

The calmodulin-stimulated ATPase of maize coleoptiles is a 140000-Mr polypeptide.

Abstract

The calmodulin-stimulated ATPase of maize (Zea mays L.) coleoptiles has been purified by calcium-dependent binding to a calmodulin affinity column. In the presence of protease inhibitors (phenylmethylsulfonylfluoride and chymostatin) a polypeptide of relative molecular mass (Mr) 140000 (±10000) is obtained on sodium-dodecylsulphate polyacrylamide gels. This polypeptide is recognised specifically by an affinity-purified polyclonal antibody to mammalian calmodulin-stimulated calcium-pumping ATPases and is of similar Mr to the erythrocyte-membrane calcium pump (138000 Mr).

Entities: Chemical Species

Year: 1988 PMID： 24220785 DOI： 10.1007/BF00392457

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

11 in total

Review 7. Intracellular calcium homeostasis.

Authors: E Carafoli
Journal: Annu Rev Biochem Date: 1987 Impact factor: 23.643

8. Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue : I. Characterization of a Ca-Pumping ATPase Associated with the Endoplasmic Reticulum.

Authors: J L Giannini; L H Gildensoph; I Reynolds-Niesman; D P Briskin
Journal: Plant Physiol Date: 1987-12 Impact factor: 8.340

4. Calmodulin regulates the Ca(2+)-dependent slow-vacuolar ion channel in the tonoplast of Chenopodium rubrum suspension cells.

Authors: T Weiser; W Blum; F W Bentrup
Journal: Planta Date: 1991-10 Impact factor: 4.116

4 in total

The calmodulin-stimulated ATPase of maize coleoptiles is a 140000-Mr polypeptide.

1. Purification of the (Ca2+-Mg2+)-ATPase from human erythrocyte membranes using a calmodulin affinity column.

2. Assay of proteins in the presence of interfering materials.

3. Protein measurement with the Folin phenol reagent.

4. Purification and properties of an adenosine triphosphatase from sarcoplasmic reticulum.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Effects of affinity-purified antibodies on the Ca2+ pumping ATPase of erythrocyte membranes.

Review 7. Intracellular calcium homeostasis.

8. Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue : I. Characterization of a Ca-Pumping ATPase Associated with the Endoplasmic Reticulum.

9. The Ca2+-pumping ATPase of plasma membranes. Purification, reconstitution and properties.

10. Calmodulin activation of plant microsomal Ca uptake.

Review 1. Recent advances in the molecular characterization of plasma membrane Ca2+ pumps.

2. Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography

Review 3. The Ca(2+)-transport ATPases from the plasma membrane.

4. Calmodulin regulates the Ca(2+)-dependent slow-vacuolar ion channel in the tonoplast of Chenopodium rubrum suspension cells.