Ling Ding1, Jingli Ma, Qin Zhou, Jintao Wang. 1. Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China. dingling79@163.com
Abstract
OBJECTIVE: To study the effect of different concentration of folate in vitro on the proliferation and apoptosis of C33A cell with HPV negative and CaSki cell with HPV16 positive, and intreaction of folate and HPV16. METHODS: C33A and Caski cells were intervened by different concentration of folate (0.1, 1.0, 10, 50, 100, 500, 1,000 and 2,000 microg/ml). The morphological changes of cells were examined by reverse discrepancy microscope. MTT and Flow Cytometry (FCM) were employed to measure the proliferation, inhibition ratio, cell cycle and apoptosis of cells. RT-PCR was used to detect the expression of HPV16 E2/E6 oncogene. RESULTS: The effects of folate on the C33A and CaSki cell growth suppression were increased gradually with the concentration of folate increasing and cultured duration prolonging, the inhibition ratio was increased (C33A: r = 0.948, P = 0.010; CaSki: r = 0.895, P = 0.006). Adding folate could induce cell apoptosis (C33A: r = 0.989, P < 0.001; CaSki: r =0.994, P < 0.001), and there was a linear relationship, but there was no significant difference on the proliferation and apoptosis of C33A and CaSki cells effected. In addition, concentration of folate did not affect the expression of HPV16 E2/E6. CONCLUSION: Different concentration of folate inhibited cervical cancer cell proliferation and enhanced cell apoptosis, but the results do not support an interaction between folate and HPV16 in cervical cancer cell.
OBJECTIVE: To study the effect of different concentration of folate in vitro on the proliferation and apoptosis of C33A cell with HPV negative and CaSki cell with HPV16 positive, and intreaction of folate and HPV16. METHODS: C33A and Caski cells were intervened by different concentration of folate (0.1, 1.0, 10, 50, 100, 500, 1,000 and 2,000 microg/ml). The morphological changes of cells were examined by reverse discrepancy microscope. MTT and Flow Cytometry (FCM) were employed to measure the proliferation, inhibition ratio, cell cycle and apoptosis of cells. RT-PCR was used to detect the expression of HPV16 E2/E6 oncogene. RESULTS: The effects of folate on the C33A and CaSki cell growth suppression were increased gradually with the concentration of folate increasing and cultured duration prolonging, the inhibition ratio was increased (C33A: r = 0.948, P = 0.010; CaSki: r = 0.895, P = 0.006). Adding folate could induce cell apoptosis (C33A: r = 0.989, P < 0.001; CaSki: r =0.994, P < 0.001), and there was a linear relationship, but there was no significant difference on the proliferation and apoptosis of C33A and CaSki cells effected. In addition, concentration of folate did not affect the expression of HPV16 E2/E6. CONCLUSION: Different concentration of folate inhibited cervical cancer cell proliferation and enhanced cell apoptosis, but the results do not support an interaction between folate and HPV16 in cervical cancer cell.