Selective uptake of neuroactive amino acids by both oligodendrocytes and astrocytes in primary dissociated culture: a possible role for oligodendrocytes in neurotransmitter metabolism.

CNS glia may be involved in the modulation of neuronal excitability through their capacity to accumulate and metabolize neuroactive amino acids. To investigate the possible role of oligodendrocytes in amino acid neurotransmitter metabolism, we have used light microscopic autoradiography, following the uptake of 3H-labelled amino acids by dissociated cultures of neonatal mouse brain, characterized immunocytochemically using cell-type specific markers. Oligodendrocytes, recognized by their characteristic galactocerebroside membrane staining, rapidly accumulated [3H] gamma-aminobutyric acid (GABA), becoming intensely labelled over cell body and processes after short incubations. In contrast, oligodendrocytes became only lightly labelled with [3H]L-glutamate and aspartate, which preferentially labelled astrocytes. [3H]D-aspartate, a non-metabolized analogue of L-glutamate, was avidly accumulated by oligodendrocytes, labelling cell bodies and processes after short incubations, to a similar extent as GABA. Thus, oligodendrocytes possess a transport mechanism for these excitatory amino acids, but rapidly metabolize them and release the metabolites. Not only the GC-positive cells but also the GC-negative undifferentiated oligodendrocyte precursors accumulated both GABA and D-aspartate, suggesting that this may be a function expressed early in the differentiation of oligodendrocytes. Net uptake of [3H] beta-alanine and [3H]glycine by oligodendrocytes was not observed under any conditions tested. A small number of oligodendrocytes were labelled with [3H]taurine after longer incubations. The uptake of certain neuroactive amino acids is thus a property shared by astrocytes and oligodendrocytes, the latter acting in a protective fashion around neuronal perikarya and axons.