BACKGROUND: The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM measles (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with enzyme immunoassay (EIA) Enzygnost (Siemens, Marburg, Germany). METHODS: Sensitivity was examined using a panel of measles laboratory confirmed sera; specificity was addressed by testing sera from healthy populations and subjects with infections that may interfere with measles IgM serology. RESULTS: The diagnostic performances of the two assays were very similar: both Enzygnost EIA and LIAISON CLIA performed with a sensitivity of 93.7% and 98.8%, whereas the specificity was 96.8% and 97.9%, respectively. CONCLUSION: We concluded that LIAISON IgM measles can be a good alternative to the other immunoassay for laboratory measles confirmation.
BACKGROUND: The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM measles (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with enzyme immunoassay (EIA) Enzygnost (Siemens, Marburg, Germany). METHODS: Sensitivity was examined using a panel of measles laboratory confirmed sera; specificity was addressed by testing sera from healthy populations and subjects with infections that may interfere with measles IgM serology. RESULTS: The diagnostic performances of the two assays were very similar: both Enzygnost EIA and LIAISON CLIA performed with a sensitivity of 93.7% and 98.8%, whereas the specificity was 96.8% and 97.9%, respectively. CONCLUSION: We concluded that LIAISON IgM measles can be a good alternative to the other immunoassay for laboratory measles confirmation.
Authors: Juan Carlos Sanz; Mar Mosquera; Belén Ramos; Rosa Ramírez; Fernando de Ory; Juan Emilio Echevarria Journal: APMIS Date: 2010-03 Impact factor: 3.205
Authors: Jm Mayoral Cortes; E Perez Morilla; V Gallardo Garcia; Jm Navarro Mari; M Perez Ruiz; R Hermosilla; J Diaz-Borrego; E Rodriguez Romero; J Ruiz Fernandez Journal: Euro Surveill Date: 2012-10-18