Literature DB >> 2421721

Rapid purification of protein kinase C by high performance liquid chromatography.

U Kikkawa, M Go, J Koumoto, Y Nishizuka.   

Abstract

Protein kinase C was purified from rat brain cytosol by using a high performance liquid chromatography (HPLC), Pharmacia FPLC system. This procedure employed a column chromatography on DE-52, followed by three steps of HPLC procedures with threonine-Sepharose (prepared as described in this report), TSK gel Phenyl-5PW (Toyo Soda), and TSK gel G3000SW (Toyo Soda) columns. Starting from about 30 g of rat brain, approximately 200 micrograms of pure enzyme was obtained. The procedure was very simple and highly reproducible. The enzyme thus obtained was nearly pure by silver staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In the presence of 10% (w/v) glycerol and 0.05% (w/v) Triton X-100, the enzyme could be stored at -80 degrees C for several months.

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Year:  1986        PMID： 2421721     DOI： 10.1016/0006-291x(86)90040-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  15 in total

1.  Phosphoprotein B-50: localization of proteolytic sites for S. aureus V8 protease using truncated cRNAs for cell-free translation.

Authors:  H B Nielander; A J Van Rozen; L H Schrama; M Kasparaitis; A B Oestreicher; W H Gispen; P Schotman
Journal:  J Mol Neurosci       Date:  1991       Impact factor: 3.444

2.  Protein kinase cross-talk: membrane targeting of the beta-adrenergic receptor kinase by protein kinase C.

Authors:  R Winstel; S Freund; C Krasel; E Hoppe; M J Lohse
Journal:  Proc Natl Acad Sci U S A       Date:  1996-03-05       Impact factor: 11.205

3.  3H-PDBu binding after administration of methylmercury to mice.

Authors:  H Katsuyama; K Saijoh; Y Inoue; K Sumino
Journal:  Bull Environ Contam Toxicol       Date:  1989-12       Impact factor: 2.151

4.  Phosphorylation of diacylglycerol kinase in vitro by protein kinase C.

Authors:  H Kanoh; K Yamada; F Sakane; T Imaizumi
Journal:  Biochem J       Date:  1989-03-01       Impact factor: 3.857

5.  Phosphorylation of human interleukin-2 (IL-2).

Authors:  H F Kung; I Calvert; E Bekesi; F R Khan; K P Huang; S Oroszlan; L E Henderson; T D Copeland; R C Sowder; S J Wei
Journal:  Mol Cell Biochem       Date:  1989-08-15       Impact factor: 3.396

6.  An apparently novel protein of human leukocytes, reactive with an antibody to protein kinase C-gamma, is rapidly modified upon cell activation: initial characterization in neutrophils and their cytoplasts.

Authors:  J I Smallwood; S E Malawista
Journal:  Inflammation       Date:  1998-02       Impact factor: 4.092

7.  Isolation of cDNA clones encoding protein kinase C: evidence for a protein kinase C-related gene family.

Authors:  G M Housey; C A O'Brian; M D Johnson; P Kirschmeier; I B Weinstein
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

8.  The interaction of aluminium with soluble protein kinase C from mouse brain.

Authors:  H Katsuyama; K Saijoh; Y Inoue; K Sumino
Journal:  Arch Toxicol       Date:  1989       Impact factor: 5.153

9.  Introduction of the beta isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells.

Authors:  E Melloni; S Pontremoli; B Sparatore; M Patrone; F Grossi; P A Marks; R A Rifkind
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

10.  Classical conditioning induces long-term translocation of protein kinase C in rabbit hippocampal CA1 cells.

Authors:  B Bank; A DeWeer; A M Kuzirian; H Rasmussen; D L Alkon
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205
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