Literature DB >> 2421601

Acceleration of nucleic acid hybridization rate by polyethylene glycol.

R M Amasino.   

Abstract

The addition of polyethylene glycol to filter-bound nucleic acid hybridization greatly increases the hybridization rate. With single-stranded probes, the increase obtained with polyethylene glycol is significantly greater than that obtained with dextran sulfate. Additionally, polyethylene glycol is easier to manipulate and less expensive than dextran sulfate.

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Year:  1986        PMID： 2421601     DOI： 10.1016/0003-2697(86)90413-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  124 in total

1.  A DNA target of 30 bp is sufficient for RNA-directed DNA methylation.

Authors:  T Pélissier; M Wassenegger
Journal:  RNA       Date:  2000-01       Impact factor: 4.942

2.  Dissociation of cytotoxicity and DNA cleavage activity induced by topoisomerase II-reactive intercalating agents in hamster-human somatic cell hybrids.

Authors:  B S Glisson; A M Killary; P Merta; W E Ross; J Siciliano; M J Siciliano
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

3.  Cytochrome c1 from potato: a protein with a presequence for targeting to the mitochondrial intermembrane space.

Authors:  H P Braun; M Emmermann; V Kruft; U K Schmitz
Journal:  Mol Gen Genet       Date:  1992-01

4.  A transcript identified by MuA of maize is associated with Mutator activity.

Authors:  M M Qin; A H Ellingboe
Journal:  Mol Gen Genet       Date:  1990-12

5.  Expression of a rice glutelin promoter in transgenic tobacco.

Authors:  D J Leisy; J Hnilo; Y Zhao; T W Okita
Journal:  Plant Mol Biol       Date:  1990-01       Impact factor: 4.076

6.  Somatic deletion of the imprinted 11p15 region in sporadic persistent hyperinsulinemic hypoglycemia of infancy is specific of focal adenomatous hyperplasia and endorses partial pancreatectomy.

Authors:  P de Lonlay; J C Fournet; J Rahier; M S Gross-Morand; F Poggi-Travert; V Foussier; J P Bonnefont; M C Brusset; F Brunelle; J J Robert; C Nihoul-Fékété; J M Saudubray; C Junien
Journal:  J Clin Invest       Date:  1997-08-15       Impact factor: 14.808

7.  Cytokinin production by Agrobacterium and Pseudomonas spp.

Authors:  D E Akiyoshi; D A Regier; M P Gordon
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

8.  Solanum brevidens possesses a non-sucrose-inducible patatin gene.

Authors:  Z Bánfalvi; Z Kostyál; E Barta
Journal:  Mol Gen Genet       Date:  1994-11-15

9.  Molecular cloning and characterization of novel isoforms of potato ADP-glucose pyrophosphorylase.

Authors:  U La Cognata; L Willmitzer; B Müller-Röber
Journal:  Mol Gen Genet       Date:  1995-03-10

10.  Abscisic acid-insensitive mutations provide evidence for stage-specific signal pathways regulating expression of an Arabidopsis late embryogenesis-abundant (lea) gene.

Authors:  R R Finkelstein
Journal:  Mol Gen Genet       Date:  1993-04
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