Fumio Matsushita1, Toshiki Kameyama, Yuzo Kadokawa, Tohru Marunouchi. 1. Department of Biology, School of Health Science, Fujita Health University, Toyoake, Aichi, Japan; Division of Cell Biology, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake, Aichi, Japan.
Abstract
BACKGROUND: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. RESULTS: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. CONCLUSION: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation.
BACKGROUND: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. RESULTS: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. CONCLUSION: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation.
Authors: Moritz Mall; Michael S Kareta; Soham Chanda; Henrik Ahlenius; Nicholas Perotti; Bo Zhou; Sarah D Grieder; Xuecai Ge; Sienna Drake; Cheen Euong Ang; Brandon M Walker; Thomas Vierbuchen; Daniel R Fuentes; Philip Brennecke; Kazuhiro R Nitta; Arttu Jolma; Lars M Steinmetz; Jussi Taipale; Thomas C Südhof; Marius Wernig Journal: Nature Date: 2017-04-05 Impact factor: 49.962
Authors: Maggie S Matern; Beatrice Milon; Erika L Lipford; Mark McMurray; Yoko Ogawa; Andrew Tkaczuk; Yang Song; Ran Elkon; Ronna Hertzano Journal: Development Date: 2020-09-11 Impact factor: 6.868