Emmanuel Somm1, Pierre Larvaron1, Yohan van de Looij2, Audrey Toulotte1, Alexandra Chatagner1, Magali Faure3, Sylviane Métairon3, Robert Mansourian3, Frédéric Raymond3, Rolf Gruetter4, Bing Wang5, Stéphane V Sizonenko1, Petra S Hüppi1. 1. Department of Paediatrics, Division of Development and Growth, University of Geneva School of Medicine, Geneva, Switzerland. 2. 1] Department of Paediatrics, Division of Development and Growth, University of Geneva School of Medicine, Geneva, Switzerland [2] Laboratory for Functional and Metabolic Imaging, E´cole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. 3. Nestlé Research Center, Lausanne, Switzerland. 4. Laboratory for Functional and Metabolic Imaging, E´cole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. 5. 1] Nestlé Research Center, Lausanne, Switzerland [2] School of Animal and Veterinary Science, Charles Sturt University, New South Wales, Australia.
Abstract
BACKGROUND: Intrauterine growth restriction (IUGR) is a major risk factor for both perinatal and long-term morbidity. Bovine lactoferrin (bLf) is a major milk glycoprotein considered as a pleiotropic functional nutrient. The impact of maternal supplementation with bLf on IUGR-induced sequelae, including inadequate growth and altered cerebral development, remains unknown. METHODS: IUGR was induced through maternal dexamethasone infusion (100 μg/kg during last gestational week) in rats. Maternal supplementation with bLf (0.85% in food pellet) was provided during both gestation and lactation. Pup growth was monitored, and Pup brain metabolism and gene expression were studied using in vivo (1)H NMR spectroscopy, quantitative PCR, and microarray in the hippocampus at postnatal day (PND)7. RESULTS: Maternal bLf supplementation did not change gestational weight but increased the birth body weight of control pups (4%) with no effect on the IUGR pups. Maternal bLf supplementation allowed IUGR pups to recover a normalized weight at PND21 (weaning) improving catch-up growth. Significantly altered levels of brain metabolites (γ-aminobutyric acid, glutamate, N-acetylaspartate, and N-acetylaspartylglutamate) and transcripts (brain-derived neurotrophic factor (BDNF), divalent metal transporter 1 (DMT-1), and glutamate receptors) in IUGR pups were normalized with maternal bLf supplementation. CONCLUSION: Our data suggest that maternal bLf supplementation is a beneficial nutritional intervention able to revert some of the IUGR-induced sequelae, including brain hippocampal changes.
BACKGROUND: Intrauterine growth restriction (IUGR) is a major risk factor for both perinatal and long-term morbidity. Bovinelactoferrin (bLf) is a major milk glycoprotein considered as a pleiotropic functional nutrient. The impact of maternal supplementation with bLf on IUGR-induced sequelae, including inadequate growth and altered cerebral development, remains unknown. METHODS: IUGR was induced through maternal dexamethasone infusion (100 μg/kg during last gestational week) in rats. Maternal supplementation with bLf (0.85% in food pellet) was provided during both gestation and lactation. Pup growth was monitored, and Pup brain metabolism and gene expression were studied using in vivo (1)H NMR spectroscopy, quantitative PCR, and microarray in the hippocampus at postnatal day (PND)7. RESULTS: Maternal bLf supplementation did not change gestational weight but increased the birth body weight of control pups (4%) with no effect on the IUGR pups. Maternal bLf supplementation allowed IUGR pups to recover a normalized weight at PND21 (weaning) improving catch-up growth. Significantly altered levels of brain metabolites (γ-aminobutyric acid, glutamate, N-acetylaspartate, and N-acetylaspartylglutamate) and transcripts (brain-derived neurotrophic factor (BDNF), divalent metal transporter 1 (DMT-1), and glutamate receptors) in IUGR pups were normalized with maternal bLf supplementation. CONCLUSION: Our data suggest that maternal bLf supplementation is a beneficial nutritional intervention able to revert some of the IUGR-induced sequelae, including brain hippocampal changes.
Authors: Yohan van de Looij; Vanessa Ginet; Alexandra Chatagner; Audrey Toulotte; Emmanuel Somm; Petra S Hüppi; Stéphane V Sizonenko Journal: Ann Clin Transl Neurol Date: 2014-12-02 Impact factor: 4.511
Authors: Eduardo F Sanches; Yohan Van de Looij; Audrey Toulotte; Analina R da Silva; Jacqueline Romero; Stephane V Sizonenko Journal: Front Neurol Date: 2018-06-25 Impact factor: 4.003
Authors: Marefa Jahan; Susie Kracht; Yen Ho; Ziaul Haque; Birendra N Bhattachatyya; Peter C Wynn; Bing Wang Journal: PLoS One Date: 2017-10-12 Impact factor: 3.240