Literature DB >> 24213548

A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures.

T J Blom1, W Kreis, F van Iren, K R Libbenga.   

Abstract

A simple apparatus was designed to allow sedimentation of plant cells grown in batch suspensions in Erlenmeyer flasks. After sedimentation the height of the cell mass along the glass wall was measured with a ruler fixed in the apparatus. The cell volume after sedimentation, calculated from this height, appeared highly correlated with the fresh weight of cells. This result was found with eight cell lines in two Laboratories. The method proved to be very suitable to allow routinely measurement of FW without the destruction of cells, from many samples, in a short time, during each phase of the growth cycle.

Year:  1992        PMID： 24213548     DOI： 10.1007/BF00232168

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  3 in total

1.  A rapid method of determining growth characteristics of plant cell populations in batch suspension culture.

Authors:  L J Gilissen; C H Hänisch Ten Cate; B Keen
Journal:  Plant Cell Rep       Date:  1983-10       Impact factor: 4.570

Review 2.  Medicinal plant research: 1953-1987.

Authors:  V E Tyler
Journal:  Planta Med       Date:  1988-04       Impact factor: 3.352

3.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905
  3 in total
  6 in total

1.  Initiation, growth and cryopreservation of plant cell suspension cultures.

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Journal:  Nat Protoc       Date:  2011-05-05       Impact factor: 13.491

2.  Production of β-damascenone precursors in cell cultures of Vitis labruscana cv Concord grapes.

Authors:  K B Shure; T E Acree
Journal:  Plant Cell Rep       Date:  1994-05       Impact factor: 4.570

3.  Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway.

Authors:  Dario Paolo; Franca Locatelli; Eleonora Cominelli; Raul Pirona; Sara Pozzo; Giulia Graziani; Alberto Ritieni; Monica De Palma; Teresa Docimo; Marina Tucci; Francesca Sparvoli
Journal:  Int J Mol Sci       Date:  2021-11-05       Impact factor: 5.923

4.  Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity.

Authors:  Damiano Gabotti; Franca Locatelli; Erica Cusano; Elena Baldoni; Annamaria Genga; Laura Pucci; Roberto Consonni; Monica Mattana
Journal:  Molecules       Date:  2019-11-09       Impact factor: 4.411

5.  New Insight into Justicidin B Pathway and Production in Linum austriacum.

Authors:  Iride Mascheretti; Michela Alfieri; Massimiliano Lauria; Franca Locatelli; Roberto Consonni; Erica Cusano; Roméo A Dougué Kentsop; Marina Laura; Gianluca Ottolina; Franco Faoro; Monica Mattana
Journal:  Int J Mol Sci       Date:  2021-03-02       Impact factor: 5.923

6.  Activation of CYCD7;1 in the central cell and early endosperm overcomes cell-cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development.

Authors:  Emily Sornay; Céline Forzani; Manuel Forero-Vargas; Walter Dewitte; James A H Murray
Journal:  Plant J       Date:  2015-10       Impact factor: 6.417
  6 in total

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