Literature DB >> 24213297

Negative staining of freshwater bacterioneuston sampled directly with electron microscope specimen support grids.

J A Fuerst1, A McGregor, M R Dickson.   

Abstract

A technique for observation of surface microlayer bacteria (bacterioneuston) is described, utilizing direct sampling of the air-water interface with carbon-stabilized electron microscope specimen support grids, followed by negative staining and transmission electron microscopy. The method resulted in excellent preservation of forms of microcolonial association, regular surface arrays, surface appendages, and prosthecae in the bacterioneuston of a freshwater pond.

Entities:  

Year:  1987        PMID： 24213297     DOI： 10.1007/BF02024999

Source DB:  PubMed          Journal:  Microb Ecol        ISSN： 0095-3628            Impact factor:   4.552


  19 in total

1.  Ecology, physiology, and genetics of fimbriae and pili.

Authors:  J C Ottow
Journal:  Annu Rev Microbiol       Date:  1975       Impact factor: 15.500

2.  Microcultural study of bacterial size changes and microcolony and ultramicrocolony formation by heterotrophic bacteria in seawater.

Authors:  F Torrella; R Y Morita
Journal:  Appl Environ Microbiol       Date:  1981-02       Impact factor: 4.792

3.  Surface arrays on the cell wall of Spirillum metamorphum.

Authors:  T J Beveridge; R G Murray
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

4.  Bacterioneuston examined with critical point drying and transmission electron microscopy.

Authors:  L Y Young
Journal:  Microb Ecol       Date:  1977-09       Impact factor: 4.552

5.  A membrane adsorption-SEM technique for observing neuston organisms.

Authors:  J S Maki; C C Remsen
Journal:  Microb Ecol       Date:  1983-07       Impact factor: 4.552

6.  [New methods for the observation and isolation of unusual or little known water bacteria].

Authors:  P Hirsch
Journal:  Z Allg Mikrobiol       Date:  1972

7.  Selective sorption of bacteria from seawater.

Authors:  K C Marshall; R Stout; R Mitchell
Journal:  Can J Microbiol       Date:  1971-11       Impact factor: 2.419

8.  Microbiological and chemical enrichment of freshwater-surface microlayers relative to the bulk-subsurface water.

Authors:  R F Hatcher; B C Parker
Journal:  Can J Microbiol       Date:  1974-07       Impact factor: 2.419

9.  Studies on the cell wall of Spirillum serpens. 1. Isolation and partial purification of the outermost cell wall layer.

Authors:  F L Buckmire; R G Murray
Journal:  Can J Microbiol       Date:  1970-10       Impact factor: 2.419

10.  Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis.

Authors:  J Smit; D A Grano; R M Glaeser; N Agabian
Journal:  J Bacteriol       Date:  1981-06       Impact factor: 3.490
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  2 in total

1.  Method for collecting air-water interface microbes suitable for subsequent microscopy and molecular analysis in both research and teaching laboratories.

Authors:  Margaret C Henk
Journal:  Appl Environ Microbiol       Date:  2004-04       Impact factor: 4.792

2.  Role of Caulobacter Cell Surface Structures in Colonization of the Air-Liquid Interface.

Authors:  Aretha Fiebig
Journal:  J Bacteriol       Date:  2019-08-22       Impact factor: 3.490
  2 in total

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