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Literature DB >> 24213291

Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. 'Centennial'.

Abstract

Efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar 'Centennial' on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. 'Centennial' expiants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.

Entities: Chemical Species

Year: 1992 PMID： 24213291 DOI： 10.1007/BF00233097

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

5 in total

1. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium.

Authors: B J Janssen; R C Gardner
Journal: Plant Mol Biol Date: 1990-01 Impact factor: 4.076

2. Histochemical analysis of CaMV 35S promoter-beta-glucuronidase gene expression in transgenic rice plants.

Authors: M J Battraw; T C Hall
Journal: Plant Mol Biol Date: 1990-10 Impact factor: 4.076

3. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

4. In vitro induction of androgenic haploids in Safflower (Carthamus tinctorius L.).

Authors: B Rajendra Prasad; M A Khadeer; P Seeta; S Y Anwar
Journal: Plant Cell Rep Date: 1991-05 Impact factor: 4.570

5. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors: R A Jefferson; T A Kavanagh; M W Bevan
Journal: EMBO J Date: 1987-12-20 Impact factor: 11.598

5 in total

4 in total

Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. 'Centennial'.

1. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium.

2. Histochemical analysis of CaMV 35S promoter-beta-glucuronidase gene expression in transgenic rice plants.

3. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

4. In vitro induction of androgenic haploids in Safflower (Carthamus tinctorius L.).

5. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

1. Improvements in rooting regenerated safflower (Carthamus tinctorius L.) shoots.

2. Agrobacterium tumefaciens mediated gene transfer in peanut (Arachis hypogaea L.).

3. Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting.

4. Parameters influencing Agrobacterium-mediated transformation system in safflower genotypes AKS-207 and PKV Pink.