| Literature DB >> 24211805 |
M Esakkirajan1, N M Prabhu2, C Arulvasu3, M Beulaja3, R Manikandan4, R Thiagarajan5, K Govindaraju6, D Prabhu3, D Dinesh3, G Babu3, G Dhanasekaran3.
Abstract
The compound was isolated from leaves of Cassia auriculata and its structure was characterized using high-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC-MS), UV-vis spectroscopy (UV-vis), fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, cytotoxicity, nuclear morphology and lactate dehydrogenase assay of isolated compound was tested against human colon cancer cell line HCT 15. The isolated compound, 4-(4-chlorobenzyl)-2,3,4,5,6,7-hexahydro-7-(2-ethoxyphenyl)benzo[h][1,4,7]triazecin-8(1H)-one at 25μg/ml concentration and by 48h showed 50% inhibition of human colon cancer cells (HCT 15). The results suggest that isolated compound from C. auriculata has potential to prevent colon cancer cell line.Entities:
Keywords: Anti-proliferative; Cassia auriculata; HCT 15 cell line
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Year: 2013 PMID： 24211805 DOI： 10.1016/j.saa.2013.09.102
Source DB: PubMed Journal: Spectrochim Acta A Mol Biomol Spectrosc ISSN： 1386-1425 Impact factor: 4.098