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Literature DB >> 24210004

Bridging the spectral gap in fluorescent proteins through directed evolution.

Abstract

Fluorescent proteins are used as noninvasive tags for protein trafficking, structure, and action. In this issue of Chemistry & Biology, Hoi and colleagues present a new optimized zFP538 yellow fluorescent protein called mPapaya1 that is bright, monomeric, and an excellent fusion partner for cellular proteins.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Luminescent Proteins

Year: 2013 PMID： 24210004 PMCID： PMC3856577 DOI： 10.1016/j.chembiol.2013.10.004

Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521

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References

12 in total

1. zFP538, a yellow fluorescent protein from coral, belongs to the DsRed subfamily of GFP-like proteins but possesses the unexpected site of fragmentation.

Authors: Vasily E Zagranichny; Natalia V Rudenko; Andrey Yu Gorokhovatsky; Mikhail V Zakharov; Zakhar O Shenkarev; Tamara A Balashova; Alexander S Arseniev
Journal: Biochemistry Date: 2004-04-27 Impact factor: 3.162

Bridging the spectral gap in fluorescent proteins through directed evolution.

1. zFP538, a yellow fluorescent protein from coral, belongs to the DsRed subfamily of GFP-like proteins but possesses the unexpected site of fragmentation.

2. Role of the biomolecular energy gap in protein design, structure, and evolution.

3. Photoconversion of YFP into a CFP-like species during acceptor photobleaching FRET experiments.

4. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

5. Photobleaching of YFP does not produce a CFP-like species that affects FRET measurements.

6. Photobleaching of YFP does not produce a CFP-like species that affects FRET measurements.

7. YFP photoconversion revisited: confirmation of the CFP-like species.

8. Engineered fluorescent proteins: innovations and applications.

9. zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore.

Review 10. Exploring protein fitness landscapes by directed evolution.