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Literature DB >> 2420824

Major outer membrane protein of Legionella pneumophila carries a species-specific epitope.

Abstract

A monoclonal antibody (LP3IIG2) directed against a species-specific epitope of Legionella pneumophila is available from Genetic Systems Corp., Seattle, Wash., for use as a diagnostic reagent. Outer membrane protein-rich fractions were prepared from L. pneumophila serogroups 1 to 8 by treatment of cell envelopes with 2% Triton X-100. Immunoblots of sodium dodecyl sulfate-polyacrylamide gels demonstrated that each membrane fraction contained two bands that reacted with LP3IIG2. The monoclonal antibody bound preferentially to a 26,000-molecular-weight band that appears to result from modification of the 29,000-molecular-weight major outer membrane protein.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1986 PMID： 2420824 PMCID： PMC268715 DOI： 10.1128/jcm.23.3.643-646.1986

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

2. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors: M J Osborn; J E Gander; E Parisi; J Carson
Journal: J Biol Chem Date: 1972-06-25 Impact factor: 5.157

3. Structural investigations on the 2-keto-3-deoxyoctonate region of lipopolysaccharides.

Authors: W Dröge; V Lehmann; O Lüderitz; O Westphal
Journal: Eur J Biochem Date: 1970-05-01

4. The cell envelope of the Legionnaires' disease bacterium. Morphologic and biochemical characteristics.

Authors: A R Flesher; S Ito; B J Mansheim; D L Kasper
Journal: Ann Intern Med Date: 1979-04 Impact factor: 25.391

5. Isolation and partial characterization of outer and inner membranes from encapsulated Haemophilus influenzae type b.

Authors: M R Loeb; A L Zachary; D H Smith
Journal: J Bacteriol Date: 1981-01 Impact factor: 3.490

6. A simplification of the protein assay method of Lowry et al. which is more generally applicable.

Authors: G L Peterson
Journal: Anal Biochem Date: 1977-12 Impact factor: 3.365

7. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.

Authors: C A Schnaitman
Journal: J Bacteriol Date: 1971-10 Impact factor: 3.490

8. Liquid medium for growth of Legionella pneumophila.

Authors: J D Ristroph; K W Hedlund; R G Allen
Journal: J Clin Microbiol Date: 1980-01 Impact factor: 5.948

9. Clinical utility of a monoclonal direct fluorescent reagent specific for Legionella pneumophila: comparative study with other reagents.

Authors: P H Edelstein; K B Beer; J C Sturge; A J Watson; L C Goldstein
Journal: J Clin Microbiol Date: 1985-09 Impact factor: 5.948

Major outer membrane protein of Legionella pneumophila carries a species-specific epitope.

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

2. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

3. Structural investigations on the 2-keto-3-deoxyoctonate region of lipopolysaccharides.

4. The cell envelope of the Legionnaires' disease bacterium. Morphologic and biochemical characteristics.

5. Isolation and partial characterization of outer and inner membranes from encapsulated Haemophilus influenzae type b.

6. A simplification of the protein assay method of Lowry et al. which is more generally applicable.

7. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.

8. Liquid medium for growth of Legionella pneumophila.

9. Clinical utility of a monoclonal direct fluorescent reagent specific for Legionella pneumophila: comparative study with other reagents.

10. Species specific membrane proteins of Legionellaceae.

1. Monoclonal antibodies for clinical applications. Patents and literature.

Review 2. Legionnaires disease: historical perspective.

3. Common epitope on the lipopolysaccharide of Legionella pneumophila recognized by a monoclonal antibody.

4. Monoclonal antibodies to Legionella Mip proteins recognize genus- and species-specific epitopes.

5. Characterization of the extent of a large outbreak of Legionnaires' disease by serological assays.