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Literature DB >> 2420759

Histochemical staining of cadmium thiolate clusters in livers of rats treated chronically with cadmium.

Abstract

In livers of rats exposed to varying doses of CdCl2 80-90% of the cadmium content present in the fresh tissue is retained if these livers are fixed with a neutral or acid formalin fixative. Cadmium assays during different stages of the staining procedure for protein bound disulphides show the ability of this staining to demonstrate cadmium thiolate clusters next to disulphides. The methods described may also be useful in gaining more insight in the mechanism involved in fixation and staining procedure of some other metals.

Entities: Chemical Species

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Year: 1986 PMID： 2420759 DOI： 10.1007/bf00493419

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

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References

13 in total

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Authors: H Meulenaar; C J van den Hamer; T S van den Ingh; T M Stolk
Journal: Biol Trace Elem Res Date: 1985-02 Impact factor: 3.738

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Authors: A F Morselt; D Broekaert; E J Jongstra-Spaapen; J H Copius-Peereboom-Stegeman
Journal: Arch Toxicol Date: 1984-09 Impact factor: 5.153

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Authors: M Sato; Y Nagai
Journal: Toxicol Appl Pharmacol Date: 1980-06-15 Impact factor: 4.219

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Authors: A F Morselt; C J van de Hamer; L Prinsen; E J Jongstra-Spaapen; J H Copius Peereboom-Stegeman; K S Bosch
Journal: Histochemistry Date: 1985

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Authors: J Tas
Journal: Histochem J Date: 1977-03

10. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

Authors: M N Berry; D S Friend
Journal: J Cell Biol Date: 1969-12 Impact factor: 10.539

Histochemical staining of cadmium thiolate clusters in livers of rats treated chronically with cadmium.

1. The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein).

2. Histochemical changes in protein disulphide bonds in rat liver and kidney after chronic cadmium administration, and the possible relation to metallothionein.

3. Mode of existence of cadmium in rat liver and kidney after prolonged subcutaneous administration.

4. Large increase in disulphide bonds containing cytosol proteins after chronic cadmium administration, estimated in isolated rat liver cells.

5. Copper toxicosis and tolerance in the rat. I--Changes in copper content of the liver and kidney.

6. Mobilization of aged cadmium deposits by dithiocarbamates.

7. Extent of cadmium accumulation and its effect on essential metals in liver, kidney, and body fluids.

8. Copper metallothionein, a copper-binding protein from Neurospora crassa.

9. The Alcian blue and combined Alcian blue--Safranin O staining of glycosaminoglycans studied in a model system and in mast cells.

10. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.