| Literature DB >> 2420623 |
Abstract
A flow-cytometric method for the detection of changes of cellular glutathione content in vital cells is described. The reaction is based on formation of a fluorescent product between o-phthaldialdehyde (OPT) and reduced glutathione (GSH). OPT is a more GSH-specific dye than other thiol-specific dyes (e.g., bromobimanes), because it forms a cyclic compound with GSH. Changes of GSH induced by oxidation or thiol-blocking agents are visualized in vital cells after a 5-min staining at room temperature.Entities:
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Year: 1986 PMID： 2420623 DOI： 10.1016/0014-4827(86)90082-0
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905