Literature DB >> 2420534

Immunogold labeling of metaphase cells.

P E Perry, E J Thomson.   

Abstract

A method is presented for the phenotypic identification of metaphase cells stained for chromosome aberration and SCE analysis. The cells are labeled in suspension with antibodies conjugated with colloidal gold, and then chromosome preparations are made using a cytocentrifuge. A silver development (IGSS) procedure is used to enhance the gold labeling for light microscopy. A variety of fixatives may be employed, permitting various cytogenetic and cytochemical staining procedures to be used.

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Year:  1986        PMID： 2420534     DOI： 10.1159/000132214

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  3 in total

1.  Antibodies to defined histone epitopes reveal variations in chromatin conformation and underacetylation of centric heterochromatin in human metaphase chromosomes.

Authors:  P Jeppesen; A Mitchell; B Turner; P Perry
Journal:  Chromosoma       Date:  1992-03       Impact factor: 4.316

2.  Expression of human CD antigens, including CD1 and CD25, by human x mouse interlineage leukaemia hybrids.

Authors:  G M Taylor; H Morten; T Carr; C Harrison; J Ridway; P Morris Jones
Journal:  Immunology       Date:  1987-12       Impact factor: 7.397

3.  In situ chromosome preparation technique for simultaneous cytogenetic and immunocytochemical studies on cell cultures of solid tumors.

Authors:  W Henn; J Lehr; G Unteregger; A Ermis; K D Zang
Journal:  Hum Genet       Date:  1990-10       Impact factor: 4.132
  3 in total

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