The reactions of ground state Cu(+) and Fe (+) with the 20 common amino acids.

A systematic investigation of the gas-phase reactions of Cu(+) and Fe(+) with the 20 common amino acids is reported. Metal ions are formed by laser ablation of a metal target and are trapped in the analyzer cell of a Fourier transform mass spectrometer. By using quadrupolar excitation to axialize the metal ions, tens of thousands of thermalizing collisions occur prior to their reactions with laser-desorbed amino acid neutral molecules. Amino acids with nonpolar side chains are found to be more reactive toward Cu(+) and Fe(+) than amino acids with polar side chain. Many of the nonpolar amino acids are found to undergo dissociative metal attachment with a neutral loss of 46 u. A (13)C-labeling experiment shows that the carboxyl group is lost during dissociative metal attachment to amino acids. Together these results suggest that these metal ions interact primarily with the carboxyl functional group in these molecules.