Organogenesis and a general procedure for plant regeneration from callus culture of a commercial duboisia hybrid (D. leichhardtii x D. myoporoides).

The Duboisia hybrid (D. leichhardtii x D. myoporoides) was regenerated from callus derived from shoot tips and young seeds on Murashige and Skoog (MS) medium containing 54μM 1-naphthalenacetic acid (NAA) and 1μM 6-benzylaminopurine (BA). Callus derived from young seeds was superior to shoot tip callus. Buds were induced from callus on MS medium supplemented with 22μM BA. The buds formed shoots when transferred to MS medium with 5 μM BA and 0.5μM NAA. Shoots were induced to form roots when placed on MS medium containing 25 μM indolebutyric acid (IBA). Plantlets so formed were transplanted and subsequently planted out in the open. Approximately 6 months were required for the full regenerative process. Trees so formed have been grown for 3 years, reached a height of 3 metres and flowered and fruited normally.