Literature DB >> 2420227

Silver staining of proteins in polyacrylamide gels: increased sensitivity through a combined Coomassie blue-silver stain procedure.

M R De Moreno, J F Smith, R V Smith.   

Abstract

A combined Coomassie blue-silver stain method has been developed in sodium dodecyl sulfate-polyacrylamide gels for the detection of proteins using the model compounds bovine serum albumin, lysozyme, and recombinant DNA-derived human insulin. Sensitivity was enhanced 2.2 to 8.6 times by the new method relative to that of silver staining alone. The new method may also be useful in enhancing detection sensitivities of other proteins.

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Year:  1985        PMID： 2420227     DOI： 10.1016/0003-2697(85)90206-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  23 in total

1.  Identification and molecular analysis of a 63-kilodalton stress protein from Neisseria gonorrhoeae.

Authors:  Y Pannekoek; J P van Putten; J Dankert
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

2.  Multiplicity of UDP-glucuronosyltransferases in fish. Purification and characterization of a phenol UDP-glucuronosyltransferase from the liver of a marine teleost, Pleuronectes platessa.

Authors:  D J Clarke; S G George; B Burchell
Journal:  Biochem J       Date:  1992-06-01       Impact factor: 3.857

3.  Quantitative proteomics: assessing the spectrum of in-gel protein detection methods.

Authors:  Victoria J Gauci; Elise P Wright; Jens R Coorssen
Journal:  J Chem Biol       Date:  2010-06-19

4.  Nuclear gene for mitochondrial leucyl-tRNA synthetase of Neurospora crassa: isolation, sequence, chromosomal mapping, and evidence that the leu-5 locus specifies structural information.

Authors:  C M Chow; R L Metzenberg; U L Rajbhandary
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

5.  Lectin and lectin-related proteins in lima bean (Phaseolus lunatus L.) seeds: biochemical and evolutionary studies.

Authors:  F Sparvoli; C Lanave; A Santucci; R Bollini; L Lioi
Journal:  Plant Mol Biol       Date:  2001-03       Impact factor: 4.076

6.  Novel catanionic surfactant vesicle vaccines protect against Francisella tularensis LVS and confer significant partial protection against F. tularensis Schu S4 strain.

Authors:  Katharina Richard; Barbara J Mann; Lenea Stocker; Eileen M Barry; Aiping Qin; Leah E Cole; Matthew T Hurley; Robert K Ernst; Suzanne M Michalek; Daniel C Stein; Philip Deshong; Stefanie N Vogel
Journal:  Clin Vaccine Immunol       Date:  2013-12-18

7.  Aspects of the protein and the lipid composition of myelinoid Marchi-positive bodies from mammalian spinal cord.

Authors:  O Corneliuson; C H Berthold; H Persson; P Fredman
Journal:  Neurochem Res       Date:  1988-12       Impact factor: 3.996

8.  Partial biochemical characterization of cell surface hydrophobicity and hydrophilicity of Candida albicans.

Authors:  K C Hazen; J G Lay; B W Hazen; R C Fu; S Murthy
Journal:  Infect Immun       Date:  1990-11       Impact factor: 3.441

9.  Coomassie blue as a near-infrared fluorescent stain: a systematic comparison with Sypro Ruby for in-gel protein detection.

Authors:  R Hussain Butt; Jens R Coorssen
Journal:  Mol Cell Proteomics       Date:  2013-09-16       Impact factor: 5.911

10.  Isolation and characterization of p-coumaroyl esterase from the anaerobic fungus Neocallimastix strain MC-2.

Authors:  W S Borneman; L G Ljungdahl; R D Hartley; D E Akin
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792
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