Literature DB >> 24201876

Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation.

D M Dusi1, M Dubald, E R de Almeida, L S Caldas, E S Gander.   

Abstract

A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene.

Entities:  

Year:  1993        PMID： 24201876     DOI： 10.1007/BF00232812

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  3 in total

1.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

2.  Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut).

Authors:  E S Gander; K O Holmstroem; G R De Paiva; L A De Castro; M Carneiro; M F Grossi de Sá
Journal:  Plant Mol Biol       Date:  1991-03       Impact factor: 4.076

3.  Protein fusions with the kanamycin resistance gene from transposon Tn5.

Authors:  B Reiss; R Sprengel; H Schaller
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
  3 in total
  3 in total

1.  Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].

Authors:  Xia An; Bo Wang; Lijun Liu; Hui Jiang; Jie Chen; Shengtuo Ye; Leiyu Chen; Pingan Guo; Xing Huang; Dingxiang Peng
Journal:  Mol Biol Rep       Date:  2014-02-02       Impact factor: 2.316

2.  Transgenic ramie [Boehmeria nivea (L.) Gaud.]: factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation and regeneration.

Authors:  Bo Wang; Lijun Liu; Xuxia Wang; Jinyu Yang; Zhenxia Sun; Na Zhang; Shimei Gao; Xiulong Xing; Dingxiang Peng
Journal:  Plant Cell Rep       Date:  2009-06-16       Impact factor: 4.570

3.  Transcript profiling reveals auxin and cytokinin signaling pathways and transcription regulation during in vitro organogenesis of Ramie (Boehmeria nivea L. Gaud).

Authors:  Xing Huang; Jie Chen; Yaning Bao; Lijun Liu; Hui Jiang; Xia An; Lunjin Dai; Bo Wang; Dingxiang Peng
Journal:  PLoS One       Date:  2014-11-21       Impact factor: 3.240
  3 in total

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