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Literature DB >> 24201783

Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter.

R Walden¹, I Czaja, T Schmülling, J Schell.

Abstract

Growth characteristics of tobacco protoplasts containing rolA linked to its own promoter, or the rolB, or rolC genes of Agrobacterium rhizogenes linked to the Cauliflower Mosaic Virus 35S RNA promoter were compared with those from untransformed plants. RolA protoplasts require auxin and cytokinin for callus formation. Protoplasts overexpressing rolB and C form callus in the absence of exogenously applied auxin and cytokinin, respectively. Long term callus growth requires auxin, but the requirement for cytokinin is not critical. Optimal transient expression of an auxin responsive promoter element occurred at lower external levels of auxin in rolB and rolC protoplasts compared with untransformed protoplasts. Addition of putrescine was required for auxin responsive transient gene expression in rolA protoplasts suggesting that polyamines, or their products affect gene expression in rolA plants.

Entities: Chemical Species

Year: 1993 PMID： 24201783 DOI： 10.1007/BF00233058

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

11 in total

1. Anther-specific expression of the rolB gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth.

Authors: A Spena; J J Estruch; E Prinsen; W Nacken; H Van Onckelen; H Sommer
Journal: Theor Appl Genet Date: 1992-08 Impact factor: 5.699

2. Single rol Genes from the Agrobacterium rhizogenes T(L)-DNA Alter Some of the Cellular Responses to Auxin in Nicotiana tabacum.

Authors: C Maurel; H Barbier-Brygoo; A Spena; J Tempé; J Guern
Journal: Plant Physiol Date: 1991-09 Impact factor: 8.340

3. High Sensitivity to Auxin is a Common Feature of Hairy Root.

Authors: W H Shen; E Davioud; C David; H Barbier-Brygoo; J Tempé; J Guern
Journal: Plant Physiol Date: 1990-10 Impact factor: 8.340

4. Inhibition of 2,4-dichlorophenoxyacetic Acid conjugation to amino acids by treatment of cultured soybean cells with cytokinins.

Authors: M J Montague; R K Enns; N R Siegel; E G Jaworski
Journal: Plant Physiol Date: 1981-04 Impact factor: 8.340

5. An Auxin-Responsive Promoter Is Differentially Induced by Auxin Gradients during Tropisms.

Authors: Y. Li; G. Hagen; T. J. Guilfoyle
Journal: Plant Cell Date: 1991-11 Impact factor: 11.277

6. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors: I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal: Plant Mol Biol Date: 1987-09 Impact factor: 4.076

4. A putative rolB gene homologue of the Agrobacterium rhizogenes TR-DNA has different morphogenetic activity in tobacco than rolB.

Authors: K Lemcke; T Schmülling
Journal: Plant Mol Biol Date: 1998-03 Impact factor: 4.076

5. Production and identification of somatic hybrids between Solanum tuberosum and S. papita by using the rolC gene as a morphological selectable marker.

Authors: C Kaendler; M Fladung; H Uhrig
Journal: Theor Appl Genet Date: 1996-03 Impact factor: 5.699

5 in total

Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter.

1. Anther-specific expression of the rolB gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth.

2. Single rol Genes from the Agrobacterium rhizogenes T(L)-DNA Alter Some of the Cellular Responses to Auxin in Nicotiana tabacum.

3. High Sensitivity to Auxin is a Common Feature of Hairy Root.

4. Inhibition of 2,4-dichlorophenoxyacetic Acid conjugation to amino acids by treatment of cultured soybean cells with cytokinins.

5. An Auxin-Responsive Promoter Is Differentially Induced by Auxin Gradients during Tropisms.

6. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

7. Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants.

8. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

9. The protein encoded by the rolB plant oncogene hydrolyses indole glucosides.

10. The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates.

1. Lipochitooligosaccharide-induced tobacco cells release a peptide as mediator of the glycolipid signal.

2. Promoter analysis of the auxin-regulated tobacco glutathione S-transferase genes Nt103-1 and Nt103-35.

3. Convergent pathways for lipochitooligosaccharide and auxin signaling in tobacco cells.

4. A putative rolB gene homologue of the Agrobacterium rhizogenes TR-DNA has different morphogenetic activity in tobacco than rolB.

5. Production and identification of somatic hybrids between Solanum tuberosum and S. papita by using the rolC gene as a morphological selectable marker.