Literature DB >> 24201727

An enzyme-immunoassay of abscisic acid in potato (Solanum commersonii) cultured cells.

S B Ryu1, P H Li, M L Brenner.   

Abstract

Estimation of abscisic acid (ABA) content in potato (Solanum commersonii) suspension-cultured cells with an enzyme-immunoassay (EIA) was investigated. In crude extracts of potato cultured cells or even after simple clean-up using C18 cartridge, EIA based on commercial monoclonal antibodies (Idetek, Inc) failed to detect any ABA content. An interference could be removed by partitioning against ethyl acetate after the C18 cartridge so that the EIA yielded an estimate of ABA similar to that determined by high pressure liquid chromatography and gas chromatography-mass spectrometry analysis. These results demonstrate the presence of metabolites in potato cultured cell extract that prevent the binding of ABA to its binding site but not the binding of tracer.

Entities:  

Year:  1992        PMID： 24201727     DOI： 10.1007/BF00232419

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  10 in total

1.  Changes after Decapitation in Concentrations of Indole-3-Acetic Acid and Abscisic Acid in the Larger Axillary Bud of Phaseolus vulgaris L. cv Tender Green.

Authors:  G F Gocal; R P Pharis; E C Yeung; D Pearce
Journal:  Plant Physiol       Date:  1991-02       Impact factor: 8.340

2.  Abscisic Acid and its relationship to seed filling in soybeans.

Authors:  J R Schussler; M L Brenner; W A Brun
Journal:  Plant Physiol       Date:  1984-10       Impact factor: 8.340

3.  Water-stress-induced changes in the abscisic acid content of guard cells and other cells of Vicia faba L. leaves as determined by enzyme-amplified immunoassay.

Authors:  M J Harris; W H Outlaw; R Mertens; E W Weiler
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

4.  Abscisic Acid ELISA: Organic Acid Interference.

Authors:  H Belefant; F Fong
Journal:  Plant Physiol       Date:  1989-12       Impact factor: 8.340

5.  ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars.

Authors:  M Walker-Simmons
Journal:  Plant Physiol       Date:  1987-05       Impact factor: 8.340

6.  The development of an indirect enzyme linked immunoassay for abscisic Acid.

Authors:  G S Ross; P A Elder; J A McWha; D Pearce; R P Pharis
Journal:  Plant Physiol       Date:  1987-09       Impact factor: 8.340

7.  Involvement of abscisic Acid in potato cold acclimation.

Authors:  H H Chen; P H Li; M L Brenner
Journal:  Plant Physiol       Date:  1983-02       Impact factor: 8.340

8.  Radioimmunoassays for the differential and direct analysis of free and conjugated abscisic acid in plant extracts.

Authors:  E W Weiler
Journal:  Planta       Date:  1980-04       Impact factor: 4.116

9.  Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture.

Authors:  P C Morris; E W Weiler; S E Maddock; M G Jones; J R Lenton; D J Bowles
Journal:  Planta       Date:  1988-01       Impact factor: 4.116

10.  A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves.

Authors:  S A Quarrie; P N Whitford; N E Appleford; T L Wang; S K Cook; I E Henson; B R Loveys
Journal:  Planta       Date:  1988-03       Impact factor: 4.116
  10 in total

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