Literature DB >> 24200788

A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426.

Hirokazu Suzuki1, Keisuke Wada, Megumi Furukawa, Katsumi Doi, Toshihisa Ohshima.   

Abstract

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

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Year:  2013        PMID： 24200788     DOI： 10.1271/bbb.130492

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  8 in total

1.  Unique plasmids generated via pUC replicon mutagenesis in an error-prone thermophile derived from Geobacillus kaustophilus HTA426.

Authors:  Jyumpei Kobayashi; Misaki Tanabiki; Shohei Doi; Akihiko Kondo; Takashi Ohshiro; Hirokazu Suzuki
Journal:  Appl Environ Microbiol       Date:  2015-08-28       Impact factor: 4.792

2.  A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species.

Authors:  Ryota Kurashiki; Tatsuki Mizuno; Kurumi Murata; Takashi Ohshiro; Hirokazu Suzuki
Journal:  Extremophiles       Date:  2019-11-07       Impact factor: 2.395

3.  Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins.

Authors:  Hirokazu Suzuki; Yuta Okumura; Yui Mikawa; Mao Takata; Shunsuke Yoshimura; Takashi Ohshiro
Journal:  Extremophiles       Date:  2021-06-30       Impact factor: 2.395

4.  Thermoadaptation-directed enzyme evolution in an error-prone thermophile derived from Geobacillus kaustophilus HTA426.

Authors:  Hirokazu Suzuki; Jyumpei Kobayashi; Keisuke Wada; Megumi Furukawa; Katsumi Doi
Journal:  Appl Environ Microbiol       Date:  2014-10-17       Impact factor: 4.792

5.  New Platform for Screening Genetic Libraries at Elevated Temperatures: Biological and Genomic Information and Genetic Tools of Geobacillus thermodenitrificans K1041.

Authors:  Kosuke Koyama; Yui Mikawa; Shota Nakagawa; Ryota Kurashiki; Takashi Ohshiro; Hirokazu Suzuki
Journal:  Appl Environ Microbiol       Date:  2022-09-07       Impact factor: 5.005

6.  A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus.

Authors:  Megumi Miyano; Kosei Tanaka; Shu Ishikawa; Kotaro Mori; Andrés Miguel-Arribas; Wilfried J J Meijer; Ken-Ichi Yoshida
Journal:  Microb Cell Fact       Date:  2018-08-17       Impact factor: 5.328

7.  Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426.

Authors:  Hirokazu Suzuki; Tatsunari Taketani; Misaki Tanabiki; Misaki Ohara; Jyumpei Kobayashi; Takashi Ohshiro
Journal:  Front Microbiol       Date:  2021-03-24       Impact factor: 5.640

8.  A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation.

Authors:  Kotaro Mori; Kaho Fukui; Ryotaro Amatsu; Shu Ishikawa; Valeria Verrone; Anil Wipat; Wilfried J J Meijer; Ken-Ichi Yoshida
Journal:  Microb Cell Fact       Date:  2022-03-08       Impact factor: 5.328
  8 in total

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