Literature DB >> 242005

31P magnetic resonance of tRNA.

M Guéron, R G Shulman.   

Abstract

We have observed well-resolved 31P resonances at 65 kG (109 MHz) in solutions of Escherichia coli tRNAGlu and yeast tRNAPhe. One resolved resonance, identified as the terminal phosphate, titrates with a pK = 6.35. Upon melting the yeast tRNAPhe in 0.1 M NaCl, without Mg++, the resolved peaks broaden and disappear in the vicinity of 35 degrees while the central cluster narrows drastically, shifts slightly, and loses its structure. Addition of Mg++ shifts one resolved peak and changes the shape of the cluster. The 31P lines are broader when observed at 65 kG than at 24 kG. The broadening is shown to come from chemical shift anisotropy which is estimated to be about 140 ppm.

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Year:  1975        PMID： 242005      PMCID： PMC433018          DOI： 10.1073/pnas.72.9.3482

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  8 in total

1.  31P magnetic resonance of purified tRNA.

Authors:  M Guéron
Journal:  FEBS Lett       Date:  1971-12-15       Impact factor: 4.124

2.  NUCLEAR MAGNETIC RESONANCE OF PHOSPHORUS IN DEOXYTHYMIDINE POLYNUCLEOTIDES.

Authors:  M MANDEL; J W WESTLEY
Journal:  Nature       Date:  1964-07-18       Impact factor: 49.962

3.  The conformational transitions in yeast tRNAPhe as studied with tRNAPhe fragments.

Authors:  D Riesner; G Maass; R Thiebe; P Philippsen; H G Zachau
Journal:  Eur J Biochem       Date:  1973-07-02

4.  High resolution NMR study of the melting of yeast tRNA Phe.

Authors:  C W Hilbers; R G Shulman; S H Kim
Journal:  Biochem Biophys Res Commun       Date:  1973-12-10       Impact factor: 3.575

5.  Frequency dependence of 31P NMR linewidths in sonicated phospholipid vesicles: effects of chemical shift anisotropy.

Authors:  J A Berden; P R Cullis; D I Hoult; A C McLaughlin; G K Radda; R E Richards
Journal:  FEBS Lett       Date:  1974-09-15       Impact factor: 4.124

6.  Peptide antibiotic-dinucleotide interactions. Nuclear magnetic resonance investigations of complex formation between actinomycin D and d-pGpC in aqueous solution.

Authors:  D J Patel
Journal:  Biochemistry       Date:  1974-05-21       Impact factor: 3.162

7.  Conformational differences between the biologically active and inactive forms of a transfer ribonucleic acid.

Authors:  A Adams; T Lindahl; J R Fresco
Journal:  Proc Natl Acad Sci U S A       Date:  1967-06       Impact factor: 11.205

8.  31P-NMR studies of tRNA.

Authors:  L M Weiner; J M Backer; A I Rezvukhin
Journal:  FEBS Lett       Date:  1974-04-15       Impact factor: 4.124
  8 in total
  14 in total

1.  Adenine nucleotide storage and secretion in platelets as studied by 31P nuclear magnetic resonance.

Authors:  K Ugurbil; H Holmsen; R G Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

2.  Asymmetric binding of the inhibitor di(adenosine-5') pentaphosphate (Ap5A) to adenylate kinase.

Authors:  B D Nageswara Rao; M Cohn
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

3.  Deploying RNA and DNA with Functionalized Carbon Nanotubes.

Authors:  Simone Alidori; Karim Asqiriba; Pablo Londero; Magnus Bergkvist; Marco Leona; David A Scheinberg; Michael R McDevitt
Journal:  J Phys Chem C Nanomater Interfaces       Date:  2013-03-21       Impact factor: 4.126

4.  High resolution 31P nuclear magnetic resonance studies of intact yeast cells.

Authors:  J M Salhany; T Yamane; R G Shulman; S Ogawa
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

5.  31P NMR studies of the solution structure and dynamics of nucleosomes and DNA.

Authors:  L Klevan; I M Armitage; D M Crothers
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

6.  Steroid diamine-nucleic acid interactions: partial insertion of dipyrandium between unstacked base pairs of the poly(dA-dT) duplex in solution.

Authors:  D J Patel; L L Canuel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

7.  A study of the conformation of 5S RNA by 31P NMR.

Authors:  P Zhang; R Rycyna; P B Moore
Journal:  Nucleic Acids Res       Date:  1989-09-25       Impact factor: 16.971

8.  Phosphorus-31 NMR studies of E. coli ribosomes.

Authors:  T R Tritton; I M Armitage
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

9.  "Alternating B-DNA" conformation for the oligo(dG-dC) duplex in high-salt solution.

Authors:  D J Patel; L L Canuel; F M Pohl
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

10.  A conformational study of nucleic acid phosphate ester bonds using phosphorus-31 nuclear magnetic resonance.

Authors:  C A Haasnoot; C Altona
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971
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