Literature DB >> 24197507

Purification and translation of ovalbumin, conalbumin, ovomucoid and lysozyme messenger RNA.

R D Palmiter1, L T Smith.   

Abstract

Messenger RNAs for 4 egg white proteins (ovalbumin, conalbumin, ovomucoid and lysozyme) were assayed in a cell-free, protein-synthesizing system derived from rabbit reticulocytes. Each of these messengers was purified about 33-fold from hen oviduct polysomal RNA using oligo(dT) cellulose. The apparent minimal translational efficiencies varied from 22 translations for each ovalbumin mRNA to less than 1 for lysozyme mRNA. These messengers are not polycistronic with each other since they have distinct sedimentation values of: lysozyme, 8.5S; ovomucoid, 11S; ovalbumin, 15S; and conalbumin 18S. Several aspects of this system indicate that it will be valuable for dissecting the fine control of mRNA metabolism.

Entities:  

Year:  1973        PMID： 24197507     DOI： 10.1007/BF00357152

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  8 in total

1.  Regulation of protein synthesis in chick oviduct. I. Independent regulation of ovalbumin, conalbumin, ovomucoid, and lysozyme induction.

Authors:  R D Palmiter
Journal:  J Biol Chem       Date:  1972-10-25       Impact factor: 5.157

2.  Purification of ovalbumin messenger ribonucleic acid by specific immunoadsorption of ovalbumin-synthesizing polysomes and millipore partition of ribonucleic acid.

Authors:  R Palacios; D Sullivan; N M Summers; M L Kiely; R T Schimke
Journal:  J Biol Chem       Date:  1973-01-25       Impact factor: 5.157

3.  Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.

Authors:  R Palacios; R D Palmiter; R T Schimke
Journal:  J Biol Chem       Date:  1972-04-25       Impact factor: 5.157

4.  Regulation of protein synthesis in chick oviduct. IV, Role of testosterone.

Authors:  R D Palmiter; M E Haines
Journal:  J Biol Chem       Date:  1973-03-25       Impact factor: 5.157

5.  Ovalbumin messenger ribonucleic acid translation. Comparable rates of polypeptide initiation and elongation on ovalbumin and globin messenger ribonucleic acid in a rabbit reticulocyte lysate.

Authors:  R D Palmiter
Journal:  J Biol Chem       Date:  1973-03-25       Impact factor: 5.157

6.  Estrogen-induced synthesis of ovalbumin messenger RNA and its translation in a cell-free system.

Authors:  G C Rosenfeld; J P Comstock; A R Means; B W O'Malley
Journal:  Biochem Biophys Res Commun       Date:  1972-02-25       Impact factor: 3.575

7.  Regulation of protein synthesis in chick oviduct. II. Modulation of polypeptide elongation and initiation rates by estrogen and progesterone.

Authors:  R D Palmiter
Journal:  J Biol Chem       Date:  1972-11-10       Impact factor: 5.157

8.  Synthesis of ovalbumin in a rabbit reticulocyte cell-free system programmed with hen oviduct ribonucleic acid.

Authors:  R E Rhoads; G S McKnight; R T Schimke
Journal:  J Biol Chem       Date:  1971-12-10       Impact factor: 5.157
  8 in total

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