Cleavage of bacteriophage M13 DNA by Haemophilus influenzae endonuclease-R.

The restriction enzyme from Haemophilus influenzae, endonuclease-R, has only one cleavage site on the double-stranded replicative form DNA of bacteriophage M13. Circular replicative forms are broken to yield full-length liniar M13-DNA molecules (RF-III). The cleavage site appears to be specific as the RF-III molecules, produced by endonuclease-R, cannot be circularized by denaturation and renaturation.