Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitors.

(3)H-puromycin binds to bacterial ribosomes, in the presence of ethanol, under the experimental conditions of the fragment reaction assay. The binding is feeble, takes place at 0°C, is partially inhibited by chloramphenicol and lincomycin and totally by sparsomycin. (3)H-puromycin binding is hardly affected by the 3' aminonucleoside of puromycin, is well inhibited by the L-Phenylalanine and L-Leucine analogues of puromycin and totally blocked by L-Phenylalanyl-adenosine.