Development of biosensors for the detection of mercury and copper ions.

The development of genetically engineered biosensors for copper and mercury ions is described. The biosensors have been constructed by fusing thelux or light emitting genes fromVibrio fischeri with genetic regulating elements that respond to copper ions or mercury ions, derived respectively fromEscherichia coli andSerratia marcescens. The fusions were placed intoE. coli cells which then emitted light in response to copper or mercury ions. Data is presented describing the sensitivity, specificity, and dynamic range of the biosensors to their respective target metal ions. A preliminary description of experiments is provided indicating how these biosensors might be used to investigate the bioavailability of mercury and copper ions in environmental samples.