Literature DB >> 24196039

Identification of a RAPD marker linked to the oat stem rust gene Pg3.

G A Penner1, J Chong, M Lévesque-Lemay, S J Molnar, G Fedak.   

Abstract

The feasibility of identifying molecular markers linked to disease resistance genes in oats was investigated utilizing random primers in conjunction with polymerase chain reaction technology. A pair of near-isogenic oat lines were screened for polymorphic DNA fragments linked to the stem rust resistance gene Pg3. Two primers were identified which amplified DNA fragments that were polymorphic between the lines analyzed. One primer (ACOpR-2) was shown to be completely linked to the Pg3 locus; the other primer was not linked to either the ACOpR-2 or the Pg3 loci. This type of analysis, combined with rapid leaf disc DNA extraction techniques, offers an effective means of identifying useful molecular markers and of applying them to plant breeding selection strategies.

Entities:  

Year:  1993        PMID： 24196039     DOI： 10.1007/BF00225008

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  6 in total

1.  A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.

Authors:  K Edwards; C Johnstone; C Thompson
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

2.  Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the tm-2a gene in tomato.

Authors:  N D Young; D Zamir; M W Ganal; S D Tanksley
Journal:  Genetics       Date:  1988-10       Impact factor: 4.562

3.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

4.  Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenic lines.

Authors:  I Paran; R Kesseli; R Michelmore
Journal:  Genome       Date:  1991-12       Impact factor: 2.166

5.  Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

Authors:  G B Martin; J G Williams; S D Tanksley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

6.  An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2.

Authors:  M Sarfatti; J Katan; R Fluhr; D Zamir
Journal:  Theor Appl Genet       Date:  1989-11       Impact factor: 5.699
  6 in total
  15 in total

1.  Mapping of the Rf-3 nuclear fertility-restoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers.

Authors:  G Zhang; Y Lu; T S Bharaj; S S Virmani; N Huang
Journal:  Theor Appl Genet       Date:  1997-01       Impact factor: 5.699

2.  The use of bulk segregant analysis to identify a RAPD marker linked to leaf rust resistance in barley.

Authors:  D M Poulsen; R J Henry; R P Johnston; J A Irwin; R G Rees
Journal:  Theor Appl Genet       Date:  1995-07       Impact factor: 5.699

3.  DNA markers tightly linked to a gall midge resistance gene (Gm2) are potentially useful for marker-aided selection in rice breeding.

Authors:  S Nair; J S Bentur; U P Rao; M Mohan
Journal:  Theor Appl Genet       Date:  1995-07       Impact factor: 5.699

4.  Identification of RAPD markers linked to a fertility restorer gene for theOgura radish cytoplasmic male sterility of rapeseed (Brassica napus L.).

Authors:  R Delourme; A Bouchereau; N Hubert; M Renard; B S Landry
Journal:  Theor Appl Genet       Date:  1994-08       Impact factor: 5.699

5.  Identification of a codominant amplified polymorphic DNA marker linked to the verticillium wilt resistance gene in tomato.

Authors:  L M Kawchuk; D R Lynch; J Hachey; P S Bains; F Kulcsar
Journal:  Theor Appl Genet       Date:  1994-11       Impact factor: 5.699

6.  RFLP markers linked to scald (Rhynchosporium secalis) resistance gene Rh2 in barley.

Authors:  G F Schweizer; M Baumer; G Daniel; H Rugel; M S Röder
Journal:  Theor Appl Genet       Date:  1995-06       Impact factor: 5.699

7.  Random amplified polymorphic DNA (RAPD) detection of dwarf off-types in micropropagated Cavendish (Musa spp. AAA) bananas.

Authors:  O P Damasco; G C Graham; R J Henry; S W Adkins; M K Smiths; I D Godwin
Journal:  Plant Cell Rep       Date:  1996-11       Impact factor: 4.570

8.  Association of a DNA marker with Hessian fly resistance gene H9 in wheat.

Authors:  I Dweikat; H Ohm; S Mackenzie; F Patterson; S Cambron; R Ratcliffe
Journal:  Theor Appl Genet       Date:  1994-12       Impact factor: 5.699

9.  Detection of the apomictic mode of reproduction in maize-Tripsacum hybrids using maize RFLP markers.

Authors:  O Leblanc; D Grimanelli; D González-de-León; Y Savidan
Journal:  Theor Appl Genet       Date:  1995-06       Impact factor: 5.699

10.  Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat.

Authors:  G M Schachermayr; M M Messmer; C Feuillet; H Winzeler; M Winzeler; B Keller
Journal:  Theor Appl Genet       Date:  1995-06       Impact factor: 5.699
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