Stimulation of cellular proliferation and differentiation in the intact and regenerating planarian Dugesia(G) tigrina by the neuropeptide substance P.

The neuropeptide substance P (SP) is shown to be a potent mitogen for intact and regenerating planarians. At nanomolar concentrations, SP markedly enhances cellular proliferation causing an increase in the mitotic index and in the number of blastema cells. Moreover, albeit to a lower extent, SP enhances cellular differentiation as shown by the increases in eye and pharynx length in regenerating organisms. On the basis of these observations, we hypothesize that SP may be one of the postulated growth factors necessary for the stimulation of proliferation, and to a lesser extent differentiation of cells in intact and regenerating planarians.