Literature DB >> 24194178

Amino acid uptake in plasma membrane vesicles isolated from proliferating tumor cells and tissues.

M G Leonardi1, R Comolli.   

Abstract

The transport of L-alanine, a natural substrate of system A, across plasma membrane vesicle preparations has been studied in the early stages of rat DENA-PH hepato-carcinogenesis and in a very undifferentiated rat ascites hepatoma cell line (Yoshida AH-130) in the exponential and stationary phase of growth.Kinetic analyses indicated an increase of the Vmax value in DENA-PH-treated rats 30 h after partial hepatectomy as well as in exponential growing Yoshida ascites cells. In DENA-PH-treated rats the Km value was drastically reduced 7 and 60 days after surgery, when enzyme-altered hyperplastic and preneoplastic lesions were present in rat liver. Drastically reduced Km values were also found in Yoshida ascites cells.The results suggest that an altered alanine transporter might take place in liver plasma membranes from carcinogen-treated rats. This appears to occur also in an established tumor cell line, grown in vivo.

Entities:  

Year:  1991        PMID： 24194178     DOI： 10.1007/BF00814007

Source DB:  PubMed          Journal:  Amino Acids        ISSN： 0939-4451            Impact factor:   3.520


  23 in total

1.  Growth-dependent regulation of system A in SV40-transformed fetal rat hepatocytes.

Authors:  M E Handlogten; M S Kilberg
Journal:  Am J Physiol       Date:  1988-09

Review 2.  The regulation of amino acid transport in animal cells.

Authors:  G G Guidotti; A F Borghetti; G C Gazzola
Journal:  Biochim Biophys Acta       Date:  1978-12-15

3.  Synthesis of native 60S and 40S ribosomal subunits in Yoshida rat ascites hepatoma AH-130 cells: correlation with the rate of cell growth.

Authors:  R Comolli; L Rusconi
Journal:  Cell Biol Int Rep       Date:  1983-02

4.  Evidence for inherent differences in the system A carrier from normal and transformed liver tissue. Differential inactivation and substrate protection in membrane vesicles and reconstituted proteoliposomes.

Authors:  K L Dudeck; E E Dudenhausen; T C Chiles; P Fafournoux; M S Kilberg
Journal:  J Biol Chem       Date:  1987-09-15       Impact factor: 5.157

5.  Rapid emergence of carcinogen-induced hyperplastic lesions in a new model for the sequential analysis of liver carcinogenesis.

Authors:  D B Solt; A Medline; E Farber
Journal:  Am J Pathol       Date:  1977-09       Impact factor: 4.307

6.  Parallel changes in amino acid transport and protein kinase C localization in LLC-PK1 cells treated with TPA or diradylglycerols.

Authors:  W D Dawson; J S Cook
Journal:  J Cell Physiol       Date:  1987-07       Impact factor: 6.384

7.  A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting Ca2+-transport and Ca2+-ATPase activities.

Authors:  R J Epping; F L Bygrave
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

8.  Cell density and amino acid transport in 3T3, SV3T3, and SV3T3 revertant cells.

Authors:  A F Borghetti; G Piedimonte; M Tramacere; A Severini; P Ghiringhelli; G G Guidotti
Journal:  J Cell Physiol       Date:  1980-10       Impact factor: 6.384

9.  Histidine transport in plasma membrane vesicles from rat liver.

Authors:  M G Leonardi; R Comolli; B Giordana
Journal:  Pflugers Arch       Date:  1988-03       Impact factor: 3.657

10.  Activation of a ribosomal S6 protein kinase in rapidly emerging diethylnitrosamine-induced gamma-glutamyltranspeptidase-positive hyperplastic liver lesions of the rat.

Authors:  R Comolli; P Alberti; G Giribaldi
Journal:  Tumori       Date:  1989-12-31
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