Regeneration in Phaseolus vulgaris L. Promotive role of N(6)-benzylaminopurine in cultures from juvenile leaves.

Plants were regenerated from cultured young leaves of Phaseolus vulgaris L. cv. Kinghorn. For inducing shoot regeneration the expiant had to consist of the petiole and a portion of the lamina, and N(6)-benzylaminopurine (BAP) had to be present in the culture medium. Furthermore, the frequency of shoot regeneration increased more than seven-fold if donor seedlings were raised on a medium containing 5 μM BAP, followed by culture of the leaf explants on a medium containing 20 μM BAP. Regenerated shoots developed roots on basal (hormone-free) medium and the resulting plantlets could be transplanted to soil.