Literature DB >> 24193786

RFLP analysis of asymmetric somatic hybrids between Solanum tuberosum and irradiated S. brevidens.

Y S Xu1, E Pehu.   

Abstract

The nuclear genome composition of five asymmetric somatic hybrids, obtained by fusion of leaf protoplasts from Solanum tuberosum and gamma-irradiated leaf protoplasts from S. brevidens, have been analyzed at the molecular level. An analysis of 21 loci using linkage group-specific restriction fragment length polymorphism (RFLP) was included in the study. All five hybrids contained a complete set of the loci studied from S. tuberosum. The degree of elimination of alleles from the irradiated S. brevidens donor genome ranged from 10-65% in the five asymmetric hybrids analyzed. The detection of incomplete chromosomes, as well as non-parental bands in Southern hybridizations with RFLP markers, revealed extensive chromosome rearrangements in the asymmetric hybrids.

Entities:  

Year:  1993        PMID： 24193786     DOI： 10.1007/BF00222666

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  17 in total

1.  High density molecular linkage maps of the tomato and potato genomes.

Authors:  S D Tanksley; M W Ganal; J P Prince; M C de Vicente; M W Bonierbale; P Broun; T M Fulton; J J Giovannoni; S Grandillo; G B Martin
Journal:  Genetics       Date:  1992-12       Impact factor: 4.562

2.  Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: Regeneration of fertile plants.

Authors:  D Dudits; E Maroy; T Praznovszky; Z Olah; J Gyorgyey; R Cella
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

3.  Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after "gamma"-fusion.

Authors:  S Hinnisdaels; L Bariller; A Mouras; V Sidorov; J Del-Favero; J Veuskens; I Negrutiu; M Jacobs
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699

4.  Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by "gamma-fusion".

Authors:  Y Y Gleba; S Hinnisdaels; V A Sidorov; V A Kaleda; A S Parokonny; N V Boryshuk; N N Cherep; I Negrutiu; M Jacobs
Journal:  Theor Appl Genet       Date:  1988-11       Impact factor: 5.699

5.  Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens.

Authors:  Y S Xu; M Murto; R Dunckley; M G Jones; E Pehu
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

6.  Restriction fragment length polymorphism analysis of somatic hybrids between Lycopersicon esculentum and irradiated L. peruvianum: evidence for limited donor genome elimination and extensive chromosome rearrangements.

Authors:  J Wijbrandi; P Zabel; M Koornneef
Journal:  Mol Gen Genet       Date:  1990-07

7.  Fertile somatic hybrids of Solanum species: RFLP analysis of a hybrid and its sexual progeny from crosses with potato.

Authors:  C E Williams; G J Hunt; J P Helgeson
Journal:  Theor Appl Genet       Date:  1990-10       Impact factor: 5.699

8.  Transfer of resistance against Phoma lingam to Brassica napus by asymmetric somatic hybridization combined with toxin selection.

Authors:  C Sjödin; K Glimelius
Journal:  Theor Appl Genet       Date:  1989-10       Impact factor: 5.699

9.  Somatic hybridization in potato: use of γ-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction.

Authors:  V A Sidorov; M K Zubko; A A Kuchko; I K Komarnitsky; Y Y Gleba
Journal:  Theor Appl Genet       Date:  1987-07       Impact factor: 5.699

10.  Intertribal hybrid cell lines of Atropa belladonna (X) Nicotiana chinensis obtained by cloning individual protoplast fusion products.

Authors:  Y Y Gleba; V P Momot; N N Cherep; M V Skarzynskaya
Journal:  Theor Appl Genet       Date:  1982-03       Impact factor: 5.699
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  2 in total

1.  Somatic hybrids between the cultivated potato Solanum tuberosum L. and the 1EBN wild species Solanum pinnatisectum Dun.: morphological and molecular characterization.

Authors:  U Menke; L Schilde-Rentschler; B Ruoss; C Zanke; V Hemleben; H Ninnemann
Journal:  Theor Appl Genet       Date:  1996-04       Impact factor: 5.699

2.  Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers.

Authors:  Paulina Smyda-Dajmund; Jadwiga Śliwka; Iwona Wasilewicz-Flis; Henryka Jakuczun; Ewa Zimnoch-Guzowska
Journal:  Plant Cell Rep       Date:  2016-03-18       Impact factor: 4.570
  2 in total

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