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Literature DB >> 2419310

Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.

Abstract

The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotriose and cycloheptaose are resistant to the action of this beta-amylase. The enzyme has a molecular weight of approximately 68,000. Restriction endonuclease mapping demonstrated that the DNA inserted in pBD64 and containing the gene is approximately 3 kilobases in length.

Entities: Chemical Gene Species

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Year: 1986 PMID： 2419310 PMCID： PMC214501 DOI： 10.1128/jb.165.3.819-824.1986

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. Transduction of linked genetic characters of the host by bacteriophage P1.

Authors: E S LENNOX
Journal: Virology Date: 1955-07 Impact factor: 3.616

2. Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid in Bacillus subtilis.

Authors: T Gryczan; S Contente; D Dubnau
Journal: Mol Gen Genet Date: 1980-02

3. Characterization of Bacillus polymyxa amylase as an exo-acting(1 leads to 4)-alpha-D-glucan maltohydrolase.

Authors: J J Marshall
Journal: FEBS Lett Date: 1974-09-15 Impact factor: 4.124

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Amino acid sequence of alpha-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene.

Authors: K Takkinen; R F Pettersson; N Kalkkinen; I Palva; H Söderlund; L Kääriäinen
Journal: J Biol Chem Date: 1983-01-25 Impact factor: 5.157

5. Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.

Authors: T Kawazu; Y Nakanishi; N Uozumi; T Sasaki; H Yamagata; N Tsukagoshi; S Udaka
Journal: J Bacteriol Date: 1987-04 Impact factor: 3.490

6. A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa.

Authors: Seong-Bin Kim; Salme Timmusk
Journal: PLoS One Date: 2013-06-27 Impact factor: 3.240

6 in total

Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.

1. Transduction of linked genetic characters of the host by bacteriophage P1.

2. Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid in Bacillus subtilis.

3. Characterization of Bacillus polymyxa amylase as an exo-acting(1 leads to 4)-alpha-D-glucan maltohydrolase.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Amino acid sequence of alpha-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene.

6. On the primary structure of amylases.

Review 7. Use of plasmids from Staphylococcus aureus for cloning of DNA in Bacillus subtilis.

8. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

9. Extracellular alkaline amylase from a Bacillus species.

10. Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis.

1. Sequence of an active fragment of B. polymyxa beta amylase.

2. A single gene directs synthesis of a precursor protein with beta- and alpha-amylase activities in Bacillus polymyxa.

3. Cloning and sequencing of the gene encoding thermophilic beta-amylase of Clostridium thermosulfurogenes.

4. Transformation of Bacillus polymyxa with plasmid DNA.

5. Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.

6. A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa.